Investigation of the Effect of Astaxanthin on Autophagy in Renal Ischemia-reperfusion Modeled Rats.

Autor: Kisaoglu A; Inonu University Faculty of Medicine, Department of Anatomy, Malatya, Türkiye., Kose E; Inonu University Faculty of Medicine, Department of Anatomy, Malatya, Türkiye., Yilmaz N; Karabuk University Faculty of Medicine, Department of Anatomy, Karabuk, Türkiye., Tanbek K; Inonu University Faculty of Medicine, Department of Physiology, Malatya, Türkiye., Yildiz A; Inonu University Faculty of Medicine, Department of Histology and Embryology, Malatya, Türkiye., Yilmaz U; Karabuk University Faculty of Medicine, Department of Physiology, Karabuk, Türkiye., Cirik RH; Inonu University Faculty of Medicine, Department of Histology and Embryology, Malatya, Türkiye., Ozbag D; Adiyaman University Faculty of Medicine, Department of Anatomy, Adiyaman, Türkiye.
Jazyk: angličtina
Zdroj: Medeniyet medical journal [Medeni Med J] 2024 Jun 28; Vol. 39 (2), pp. 101-108.
DOI: 10.4274/MMJ.galenos.2024.27243
Abstrakt: Objective: The aim of this study was to investigate the effect of various astaxanthin (ATX) doses on oxidative damage and autophagy in renal ischemia-reperfusion (I/R) injury-modeled rats.
Methods: The rats were divided into five groups: sham group (n=8), I/R (n=8), I/R + 5 mg/kg ATX (n=8), I/R + 10 mg/kg ATX (n=8), and I/R + 25 mg/kg ATX (n=8) groups. ATX was dissolved in 5 mg/kg, 10 mg/kg, and 25 mg/kg olive oil for 7 days and administered to the rats in the experimental group. Sham and I/R groups were also administered ATX solution (olive oil) via oral gavage for 7 days. Renal ischemia reperfusion was induced in all rats except the sham group after the last dose was administered on the 7 th day. Reperfusion was conducted for 24 hours after 45 minutes of ischemia.
Results: Blood samples were collected, and kidney tissue were incised for biochemical and histological analyses. Superoxide dismutase (SOD) and total antioxidant status (TAS) were significantly lower in the I/R group than in the sham group (p<0.05), whereas malondialdehyde (MDA) and total oxidant status (TOS) values were higher (p<0.05). It was determined that SOD and TAS increased and MDA and TOS decreased in the ATX-administration groups compared with the I/R group, independent of the dose (p<0.05). In the 25 mg/kg ATX + I/R group, Beclin-1 and LC3β immunoreactivities were significantly higher than those in the other groups (p<0.05). The lowest p62 immunoreactivity was observed in the 25 mg/kg ATX + I/R group.
Conclusions: ATX had a protective effect on kidney function and against oxidative damage. Furthermore, high-dose ATX administration protected kidney tissue via autophagy induction in this study.
Competing Interests: Conflict of Interest: The authors have no conflict of interest to declare.
(Copyright© 2024 The Author. Published by Galenos Publishing House on behalf of Istanbul Medeniyet University Faculty of Medicine.)
Databáze: MEDLINE
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