Inhibition of Melanoma Cell Growth by Salvianolic Acid A through CHK2-CDC25A Pathway Modulation.

Autor:	Pu XY; The School of Public Health, Fujian Medical University, 350122 Fuzhou, Fujian, China.; Department of Clinical Nutrition, The Second Affiliated Hospital of Fujian Medical University, 362000 Quanzhou, Fujian, China., Mei Y; Department of Dermatology, The Second Affiliated Hospital of Fujian Medical University, 362000 Quanzhou, Fujian, China., Zheng Q; The School of Public Health, Fujian Medical University, 350122 Fuzhou, Fujian, China.; Department of Clinical Nutrition, The Second Affiliated Hospital of Fujian Medical University, 362000 Quanzhou, Fujian, China., Ko CY; Department of Clinical Nutrition, The Second Affiliated Hospital of Fujian Medical University, 362000 Quanzhou, Fujian, China.
Jazyk:	angličtina
Zdroj:	Frontiers in bioscience (Landmark edition) [Front Biosci (Landmark Ed)] 2024 Jun 12; Vol. 29 (6), pp. 213.
DOI:	10.31083/j.fbl2906213
Abstrakt:	Background: This study investigated the impact of salvianolic acids, derived from Danshen, on melanoma cell growth. Specifically, we assessed the ability of salvianolic acid A (Sal A) to modulate melanoma cell proliferation. Methods: We used human melanoma A2058 and A375 cell lines to investigate the effects of Sal A on cell proliferation and death by measuring bromodeoxyuridine incorporation and lactate dehydrogenase release. We assessed cell viability and cycle progression using water soluble tetrazolium salt-1 (WST-1) mitochondrial staining and propidium iodide. Additionally, we used a phospho-kinase array to investigate intracellular kinase phosphorylation, specifically measuring the influence of Sal A on checkpoint kinase-2 (Chk-2) via western blot analysis. Results: Sal A inhibited the growth of A2058 and A375 cells dose-responsively and induced cell cycle arrest at the G2/M phase. Notably, Sal A selectively induces Chk-2 phosphorylation without affecting Chk-1, thereby degrading Chk-2-regulated genes Cdc25A and Cdc2 . However, Sal A does not affect the Chk1-Cdc25C pathway. Conclusions: Salvianolic acids, especially Sal A, effectively hinder melanoma cell growth by inducing Chk-2 phosphorylation and disrupting G2/M checkpoint regulation. Competing Interests: The authors declare no conflict of interest. (© 2024 The Author(s). Published by IMR Press.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu