Siglec-1, an easy and contributory inflammation marker in rheumatology.

Autor: Boz V; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy., Tesser A; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy., Burlo F; Department of Medical, Surgical and Health Sciences University of Trieste Trieste Italy., Donadel N; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy., Pastore S; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy., Amaddeo A; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy., Vittoria F; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy., Padovan M; Department of Medical, Surgical and Health Sciences University of Trieste Trieste Italy., Di Rosa M; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy.; Present address: Department of Medical, Surgical and Health Sciences University of Trieste Trieste 34149 Italy., Tommasini A; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy.; Department of Medical, Surgical and Health Sciences University of Trieste Trieste Italy., Valencic E; Department of Pediatrics Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy.
Jazyk: angličtina
Zdroj: Clinical & translational immunology [Clin Transl Immunology] 2024 Jun 26; Vol. 13 (7), pp. e1520. Date of Electronic Publication: 2024 Jun 26 (Print Publication: 2024).
DOI: 10.1002/cti2.1520
Abstrakt: Objectives: Inflammatory markers such as erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) are poorly informative about interferon (IFN)-related disorders. In these conditions, the measure of the interferon score (IS), obtained by measuring the expression of IFN-stimulated genes, has been proposed. Flow cytometry-based assays measuring sialic-acid-binding Ig-like lectin 1 (Siglec-1) expression could be a more practical tool for evaluating IFN-inflammation. The study compared Siglec-1 measures with IS and other inflammatory indexes. We compared Siglec-1 measures with IS and other inflammatory indexes in real-world paediatric rheumatology experience.
Methods: We recruited patients with immuno-rheumatological conditions, acute infectious illness and patients undergoing orthopaedic surgery as controls. Siglec-1 expression was measured in all samples, and IS, ESR and CRP were also recorded if available.
Results: Overall, 98 subjects were enrolled in the study, with a total of 104 measures of Siglec-1. Compared with IS, Siglec-1 expression showed good accuracy (86.0%), specificity (72.7%) and sensitivity (85.7%). The measure of the percentage of Siglec-1-positive cells performed best at low levels of IFN-inflammation, while the measure of mean fluorescence intensity performed best at higher levels. Ex vivo studies on IFN-stimulated monocytes confirmed this behaviour. There was no link between Siglec-1 expression and either ESR or CRP, and positive Siglec-1 results were found even when ESR and CRP were normal. A high Siglec-1 expression was also recorded in subjects with acute infections.
Conclusion: Siglec-1 measurement by flow cytometry is an easy tool to detect IFN-related inflammation, even in subjects with normal results of common inflammation indexes.
Competing Interests: The authors declare no conflict of interest.
(© 2024 The Author(s). Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.)
Databáze: MEDLINE
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