A mystery revealed: an update on eosinophil and other blood cell morphology of the Argentine black and white tegu ( Salvator merianae ).

Autor:	Bosch SN; Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL, United States., Stacy NI; Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, United States., Armien AG; California Animal Health and Food Safety Laboratory Systems (CAHFS), School of Veterinary Medicine, University of California, Davis, Davis, CA, United States., Hollinger C; Inotiv, Inc., Kalamazoo, MI, United States.; Wildlife Conservation Society, Zoological Health Program, Bronx Zoo, Bronx, NY, United States., Minor R; Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL, United States., Heard DJ; Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL, United States., Stokol T; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2024 Jun 13; Vol. 11, pp. 1387178. Date of Electronic Publication: 2024 Jun 13 (Print Publication: 2024).
DOI:	10.3389/fvets.2024.1387178
Abstrakt:	Reptile white blood cell (WBC) morphological features are strikingly variable across species. In the Argentine black and white tegu ( Salvator merianae ), red tegu ( Salvator rufescens ), and Savannah monitor (Var anus exanthematicus ), previous reports described a WBC type with a single distinct, clear, linear- to ovoid- to crescent-shaped inclusion of presumptive monocytic origin. The objective of this study was to further investigate the origin of this unique WBC type with crescent-shaped inclusions. Blood samples from two Argentine black and white tegus, tegu 1, a 4-year-old female, and tegu 2, a 2-year-old presumed male, were submitted for routine hematological evaluation. Additional blood films were prepared and stained with these cytochemical stains: alkaline phosphatase (ALP; naphthol AS-MX phosphate substrate), alpha-naphthyl butyrate esterase, alpha-chloroacetate esterase, myeloperoxidase, Periodic acid-Schiff, and Sudan black B. Blood films from tegu 1 were also stained with a second ALP stain (5-bromo-4-chloro-3-indoxyl-phosphate and nitroblue tetrazolium substrate), Luna, luxol fast blue, and toluidine blue. The blood from tegu 1 was cytocentrifuged to isolate and fix the buffy coat in glutaraldehyde 2.5% aqueous solution for transmission electron microscopy. Six morphologically distinct WBC types were identified from tegu 1, including heterophils, basophils, monocytes, azurophils, lymphocytes, and the unique WBC type, which were identified as eosinophils with inclusions. WBC types in tegu 2 were similar; however, eosinophils lacked a discernable inclusion. Proper WBC identification will be useful in obtaining accurate hemogram data for this species. Competing Interests: CH was employed by Inotiv. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Bosch, Stacy, Armien, Hollinger, Minor, Heard and Stokol.)
Databáze:	MEDLINE
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