Can silver nanoparticles stabilized by Fenugreek ( Trigonella foenm -graecum ) improve tibial bone defects repair in rabbits? A preliminary study.

Autor: Sadan M; Department of Clinical Sciences, College of Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia.; Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt., Naem M; Department of Clinical Sciences, College of Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia., Tawfeek HM; Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt., Khodier MM; Department of Pathology, College of Medicine, Qassim University, Buraidah, Saudi Arabia.; Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt., Zeitoun MM; Department of Animal and Fish Production, Faculty of Agriculture, Alexandria University, Alexandria, Egypt., El-Khodery S; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt., Alkhamiss AS; Department of Pathology, College of Medicine, Qassim University, Buraidah, Saudi Arabia., Hassan YAH; Department Psychology, College of Education, Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.; Department of Psychology, College of Education, South Valley University, Qena, Egypt., Abdellatif AAH; Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraidah, Saudi Arabia.; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt.
Jazyk: angličtina
Zdroj: Open veterinary journal [Open Vet J] 2024 May; Vol. 14 (5), pp. 1281-1293. Date of Electronic Publication: 2024 May 31.
DOI: 10.5455/OVJ.2024.v14.i5.23
Abstrakt: Background: A fracture is considered a medical emergency leading to considerable complications.
Aim: This study aimed to describe the accelerating action of Ag-NPs-FG on fracture healing in rabbits.
Methods: Silver NPs (AgNPs) were reduced with fenugreek (FG), loaded into a starch gel base, and investigated for their morphology, size, and charge. Four equal groups were randomly formed of 40 adult male rabbits. A 3.5 mm diameter bone defect was created at the proximal metaphysis of the right tibia in each rabbit. Groups 1-4 were injected with placebo saline, AgNPs-FG, plain gel, and FG-gel at the bone defect zone, respectively. The healing was assessed for 8 weeks postoperatively based on the radiographic, bone turnover markers, and histopathological examinations.
Results: The AgNPs-FG was obtained as a faint reddish color, spherical in shape, with an absorbance of 423 nm, a size of 118.0 ± 1.7 nm, and a surface charge of -7.8 ± 0.518 mV. The prepared AgNPs-FG hydrogel was clear, translucent, and homogenous. The pH values were 6.55-6.5 ± 0.2, the viscosity of 4,000 and 1,875 cPs, and spreadability of 1.6 ± 0.14 and 2.0 ± 0.15 for both FG and AgNPs-FG hydrogel, respectively. The radiographic union scale was significantly ( p < 0.05) improved in group 2 with a significant ( p < 0.05) increase in bone turnover markers was found in comparison to other treated groups. Histopathological examination revealed the formation of mature bone on the 28th postoperative day in groups 2 and 4.
Conclusion: Colloidal nano-formulation of AgNPs-FG loaded hydrogel could be a promising formulation to accelerate rabbits' tibial bone healing process.
Competing Interests: The authors declare that there is no conflict of interest.
Databáze: MEDLINE
Externí odkaz: