Anticonvulsant and anxiolytic-like potential of the essential oil from the Ocimum basilicum Linn leaves and its major constituent estragole on adult zebrafish (Danio rerio).

Autor: Batista FLA; Laboratory of Pharmacology and Molecular Chemistry, Department of Chemical Biology, Regional University of Cariri (URCA), Rua Coronel Antônio Luis 1161, Pimenta, CEP 63105-000, Crato, Ceará, Brazil; Laboratory of Bioprospection of Natural Products and Biotechnology, Department of Chemistry, State University of Ceará, Campus CECITEC, Tauá, Ceará, Brazil., de Araújo SMB; Laboratory of Bioprospection of Natural Products and Biotechnology, Department of Chemistry, State University of Ceará, Campus CECITEC, Tauá, Ceará, Brazil., de Sousa DB; Laboratory of Bioprospection of Natural Products and Biotechnology, Department of Chemistry, State University of Ceará, Campus CECITEC, Tauá, Ceará, Brazil., Sobrinho FBC; Laboratory of Bioprospection of Natural Products and Biotechnology, Department of Chemistry, State University of Ceará, Campus CECITEC, Tauá, Ceará, Brazil., de Lima Silva MG; Laboratory of Pharmacology and Molecular Chemistry, Department of Chemical Biology, Regional University of Cariri (URCA), Rua Coronel Antônio Luis 1161, Pimenta, CEP 63105-000, Crato, Ceará, Brazil., de Oliveira MRC; Laboratory of Pharmacology and Molecular Chemistry, Department of Chemical Biology, Regional University of Cariri (URCA), Rua Coronel Antônio Luis 1161, Pimenta, CEP 63105-000, Crato, Ceará, Brazil., da Costa RHS; Laboratory of Pharmacology and Molecular Chemistry, Department of Chemical Biology, Regional University of Cariri (URCA), Rua Coronel Antônio Luis 1161, Pimenta, CEP 63105-000, Crato, Ceará, Brazil., Rodrigues LB; Laboratory of Pharmacology and Molecular Chemistry, Department of Chemical Biology, Regional University of Cariri (URCA), Rua Coronel Antônio Luis 1161, Pimenta, CEP 63105-000, Crato, Ceará, Brazil., Bezerra FS; Laboratory of Bioprospection of Natural Products and Biotechnology, Department of Chemistry, State University of Ceará, Campus CECITEC, Tauá, Ceará, Brazil., de Azevedo DV; Laboratory of Bioprospection of Natural Products and Biotechnology, Department of Chemistry, State University of Ceará, Campus CECITEC, Tauá, Ceará, Brazil., Vieira-Neto AE; Nucleus of Experimental Biology, University of Fortaleza, Fortaleza, Ceará, Brazil., Magalhães FEA; Laboratory of Bioprospection of Natural Products and Biotechnology, Department of Chemistry, State University of Ceará, Campus CECITEC, Tauá, Ceará, Brazil., de Menezes IRA; Laboratory of Pharmacology and Molecular Chemistry, Department of Chemical Biology, Regional University of Cariri (URCA), Rua Coronel Antônio Luis 1161, Pimenta, CEP 63105-000, Crato, Ceará, Brazil. Electronic address: irwin.alencar@urca.br.
Jazyk: angličtina
Zdroj: Neurochemistry international [Neurochem Int] 2024 Sep; Vol. 178, pp. 105796. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1016/j.neuint.2024.105796
Abstrakt: The Ocimum species present active compounds with the potential to develop drugs for treating chronic disease conditions, such as anxiety and seizures. The present study aims to investigate the anticonvulsant and anxiolytic-like effect of the essential oil from O. basilicum Linn (OEFOb) leaves and its major constituent estragole (ES) in vivo on adult zebrafish (aZF) and in silico. The aZF were treated with OEFOb or ES or vehicle and submitted to the tests of toxicity, open-field, anxiety, and convulsion and validated the interactions of the estragole on the involvement of GABAergic and serotonergic receptors by molecular docking assay. The results showed that the oral administration of OEFOb and ES did not have a toxic effect on the aZF and showed anxiolytic-like effects with the involvement of GABA A , 5-HT 1 , 5-HT 2A/2C and 5-HT 3A/3B as well on anxiety induced by alcohol withdrawal. The OEFOb and ES showed anticonvulsant potential attenuating the seizures induced by pentylenetetrazole (PTZ) by modulation of the GABA A system. Both anxiolytic and anticonvulsant effects were corroborated by the potential of the interaction of ES by in silico assay. These study samples demonstrate the pharmacological evidence and potential for using these compounds to develop new anxiolytic and anticonvulsant drugs.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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