Ultrasonic-assisted extraction of a low molecular weight polysaccharide from Nostoc commune Vaucher and its structural characterization and immunomodulatory activity.

Autor: Pan Y; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; Jilin Province Economic Management Cadre College,Changchun 130012, PR China., Liu C; Jilin Province Economic Management Cadre College,Changchun 130012, PR China., Jiang S; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China., Guan L; College of Life Sciences, Jilin Agricultural University, Changchun 130118, PR China., Liu X; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China. Electronic address: liuxinyao6660@163.com., Wen L; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China. Electronic address: wenliankui@163.com.
Jazyk: angličtina
Zdroj: Ultrasonics sonochemistry [Ultrason Sonochem] 2024 Aug; Vol. 108, pp. 106961. Date of Electronic Publication: 2024 Jun 24.
DOI: 10.1016/j.ultsonch.2024.106961
Abstrakt: In the current study, a novel crude polysaccharide (cNCEP) was extracted from N. commune Vaucher utilizing ultrasonic-assisted extraction (UAE) with 60 % ethanol, employing response surface methodology. The optimal yield of cNCEP was determined to be 8.07 ± 0.08 mg/g, achieved through ultrasonic-assisted extraction under the conditions of a material-to-liquid ratio of 1:22, temperature of 56 °C, power of 570 W, and duration of 147 min. Subsequent purification of NCEP via Sephadex G75 resulted in a novel polysaccharide with a molecular weight of 20.466 kDa. NCEP exhibited significant scavenging activites against DPPH and hydroxyl radicals, as well as notable in vitro immunomodulatory properties. Furthermore, the mechanisms underlying the immunomodulatory effects of NCEP, involving enhancement of immunity, were investigated, revealing potential regulation of MAPK and TLR4-IRF7-NF-κB signaling pathways through RNA-Seq and Western blot analyses. These findings highlight the promising potential of NCEP as an organic immunomodulatory agent and functional food ingredient.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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