Enhancing cell cryopreservation with acidic polyamino acids integrated liquid marbles.

Autor: Liu M; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China., Liang L; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China., Yu C; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China., Guo B; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China., Zhang H; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China., Yao F; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300350, China., Zhang H; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: zhanghong@tju.edu.cn., Li J; Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300350, China. Electronic address: li41308@tju.edu.cn.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Sep; Vol. 241, pp. 114055. Date of Electronic Publication: 2024 Jun 24.
DOI: 10.1016/j.colsurfb.2024.114055
Abstrakt: Cryopreservation is highly desired for long-term maintenance of the viability of living biosamples, while effective cell cryopreservation still relies heavily on the addition of dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS). However, the intrinsic toxicity of DMSO is still a bottleneck, which could not only cause the clinical side effect but also induce cell genetic variants. In the meantime, the addition of FBS may bring potentially the risk of pathogenic microorganism contamination. The liquid marbles (LMs), a novel biotechnology tool for cell cryopreservation, which not only have a small volume system that facilitated recovery, but the hydrophobic shell also resisted the harm to cells caused by adverse environments. Previous LM-based cell cryopreservation relied heavily on the addition of FBS. In this work, we introduced acidic polyaspartic acid and polyglutamic acid as cryoprotectants to construct LM systems. LMs could burst in an instant to facilitate and achieve ultrarapid recovery process, and the hydrophilic carboxyl groups of the cryoprotectants could form hydrogen bonds with water molecules and further inhibit ice growth/formation to protect cells from cryoinjuries. The L929 cells could be well cryopreserved by acidic polyamino acid-based LMs. This new biotechnology platform is expected to be widely used for cell cryopreservation, which has the potential to propel LMs for the preservation of various functional cells in the future.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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