A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans.

Autor: Fryer E; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America.; Department of Plant Biology, Carnegie Institution for Science, Stanford, California, United States of America., Guha S; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America., Rogel-Hernandez LE; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America., Logan-Garbisch T; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America.; Neurosciences Graduate Program, Stanford University, Stanford, California, United States of America., Farah H; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America.; Department of Plant Biology, Carnegie Institution for Science, Stanford, California, United States of America., Rezaei E; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America., Mollhoff IN; Department of Biology, Stanford University, Stanford, California, United States of America., Nekimken AL; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America.; Department of Mechanical Engineering, Stanford University, Stanford, California, United States of America., Xu A; Department of Plant Biology, Carnegie Institution for Science, Stanford, California, United States of America., Seyahi LS; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America.; Department of Plant Biology, Carnegie Institution for Science, Stanford, California, United States of America., Fechner S; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America., Druckmann S; Department of Neurobiology, Stanford University, Stanford, California, United States of America., Clandinin TR; Department of Neurobiology, Stanford University, Stanford, California, United States of America., Rhee SY; Department of Plant Biology, Carnegie Institution for Science, Stanford, California, United States of America., Goodman MB; Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, United States of America.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2024 Jun 27; Vol. 22 (6), pp. e3002672. Date of Electronic Publication: 2024 Jun 27 (Print Publication: 2024).
DOI: 10.1371/journal.pbio.3002672
Abstrakt: Throughout history, humans have relied on plants as a source of medication, flavoring, and food. Plants synthesize large chemical libraries and release many of these compounds into the rhizosphere and atmosphere where they affect animal and microbe behavior. To survive, nematodes must have evolved the sensory capacity to distinguish plant-made small molecules (SMs) that are harmful and must be avoided from those that are beneficial and should be sought. This ability to classify chemical cues as a function of their value is fundamental to olfaction and represents a capacity shared by many animals, including humans. Here, we present an efficient platform based on multiwell plates, liquid handling instrumentation, inexpensive optical scanners, and bespoke software that can efficiently determine the valence (attraction or repulsion) of single SMs in the model nematode, Caenorhabditis elegans. Using this integrated hardware-wetware-software platform, we screened 90 plant SMs and identified 37 that attracted or repelled wild-type animals but had no effect on mutants defective in chemosensory transduction. Genetic dissection indicates that for at least 10 of these SMs, response valence emerges from the integration of opposing signals, arguing that olfactory valence is often determined by integrating chemosensory signals over multiple lines of information. This study establishes that C. elegans is an effective discovery engine for determining chemotaxis valence and for identifying natural products detected by the chemosensory nervous system.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Fryer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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