Psidium guajav-mediated zinc oxide nanoparticles as a multifunctional, microbicidal, antioxidant and antiproliferative agent against destructive pathogens.

Autor: Thejashwini PP; Nanobiotechnology Laboratory, DOS in Biotechnology, University of Mysore, Manasagangotri, Mysuru, 570006, Karnataka, India., Chandrika R; Nanobiotechnology Laboratory, DOS in Biotechnology, University of Mysore, Manasagangotri, Mysuru, 570006, Karnataka, India., Madhusudhan MC; Nanobiotechnology Laboratory, DOS in Biotechnology, University of Mysore, Manasagangotri, Mysuru, 570006, Karnataka, India., Joshi SM; Department of Biotechnology, University of Agricultural Sciences, Dharwad, 580 005, India., Ali D; Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia., Alarifi S; Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia., Jogaiah S; Department of Environmental Science, Central University of Kerala, Tejaswini Hills, Periye (P.O), Kasaragod, Kerala, 671316, India. jsudish@cukerala.ac.in., Geetha N; Nanobiotechnology Laboratory, DOS in Biotechnology, University of Mysore, Manasagangotri, Mysuru, 570006, Karnataka, India. geetha@appbot.uni-mysore.ac.in.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2024 Sep; Vol. 47 (9), pp. 1571-1584. Date of Electronic Publication: 2024 Jun 27.
DOI: 10.1007/s00449-024-03052-x
Abstrakt: Bio-inspired zinc oxide nanoparticles are gaining immense interest due to their safety, low cost, biocompatibility, and broad biological properties. In recent years, much research has been focused on plant-based nanoparticles, mainly for their eco-friendly, facile, and non-toxic character. Hence, the current study emphasized a bottom-up synthesis of zinc oxide nanoparticles (ZnO NPs) from Psidium guajava aqueous leaf extract and evaluation of its biological properties. The structural characteristic features of biosynthesized ZnO NPs were confirmed using various analytical methods, such as UV-Vis spectroscopy, X-ray diffraction (XRD), energy-dispersive X-ray analysis (EDX), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), Scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HR-TEM). The synthesized ZnO NPs exhibited a hydrodynamic shape with an average particle size of 11.6-80.2 nm. A significant antimicrobial efficiency with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 40 and 27 µg/ml for Enterococcus faecalis, followed by 30 and 40 µg/ml for Staphylococcus aureus, 20 and 30 µg/ml for Staphylococcus mutans, 30 µg/ml for Candida albicans was observed by ZnO NPs. Additionally, they showed significant breakdown of biofilms of Streptococcus mutans and Candida albicans indicating their future value in drug-resistance research. Furthermore, an excellent dose-dependent activity of antioxidant property was noticed with an IC 50 of 9.89 µg/ml. The antiproliferative potential of the ZnO NPs was indicated by the viability of MDA MB 231 cells, which showed a drastic decrease in response to increased concentrations of biosynthesized ZnO NPs. Thus, the present results open up vistas to explore their pharmaceutical potential for the development of targeted anticancer drugs in the future.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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