Maturation of germinal center B cells after influenza virus vaccination in humans.

Autor:	McIntire KM; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA., Meng H; Department of Pathology, Yale School of Medicine, New Haven, CT, USA., Lin TH; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA, USA., Kim W; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA.; Department of Microbiology, Korea University College of Medicine, Seoul, Korea., Moore NE; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA, USA., Han J; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA, USA., McMahon M; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Wang M; Department of Pathology, Yale School of Medicine, New Haven, CT, USA.; Program in Computational Biology and Bioinformatics, Yale University , New Haven, CT, USA., Malladi SK; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA., Mohammed BM; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA., Zhou JQ; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA., Schmitz AJ; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA., Hoehn KB; Department of Pathology, Yale School of Medicine, New Haven, CT, USA., Carreño JM; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Yellin T; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Suessen T; Mallinckrodt Institute of Radiology, Washington University School of Medicine , St Louis, MO, USA., Middleton WD; Mallinckrodt Institute of Radiology, Washington University School of Medicine , St Louis, MO, USA., Teefey SA; Mallinckrodt Institute of Radiology, Washington University School of Medicine , St Louis, MO, USA., Presti RM; Department of Internal Medicine-Infectious Diseases, Washington University School of Medicine, St Louis, MO, USA.; Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine , St. Louis, MO, USA.; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine , St Louis, MO, USA., Krammer F; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai , New York, NY, USA.; Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Turner JS; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA., Ward AB; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA, USA., Wilson IA; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA, USA., Kleinstein SH; Department of Pathology, Yale School of Medicine, New Haven, CT, USA.; Program in Computational Biology and Bioinformatics, Yale University , New Haven, CT, USA.; Department of Immunobiology, Yale School of Medicine, New Haven, CT, USA., Ellebedy AH; Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA.; Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine , St. Louis, MO, USA.; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine , St Louis, MO, USA.
Jazyk:	angličtina
Zdroj:	The Journal of experimental medicine [J Exp Med] 2024 Aug 05; Vol. 221 (8). Date of Electronic Publication: 2024 Jun 27.
DOI:	10.1084/jem.20240668
Abstrakt:	Germinal centers (GC) are microanatomical lymphoid structures where affinity-matured memory B cells and long-lived bone marrow plasma cells are primarily generated. It is unclear how the maturation of B cells within the GC impacts the breadth and durability of B cell responses to influenza vaccination in humans. We used fine needle aspiration of draining lymph nodes to longitudinally track antigen-specific GC B cell responses to seasonal influenza vaccination. Antigen-specific GC B cells persisted for at least 13 wk after vaccination in two out of seven individuals. Monoclonal antibodies (mAbs) derived from persisting GC B cell clones exhibit enhanced binding affinity and breadth to influenza hemagglutinin (HA) antigens compared with related GC clonotypes isolated earlier in the response. Structural studies of early and late GC-derived mAbs from one clonal lineage in complex with H1 and H5 HAs revealed an altered binding footprint. Our study shows that inducing sustained GC reactions after influenza vaccination in humans supports the maturation of responding B cells. (© 2024 McIntire et al.)
Databáze:	MEDLINE
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