A 44-Nucleotide Region in the Chikungunya Virus 3' UTR Dictates Viral Fitness in Disparate Host Cells.

Autor: Ander SE; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA., Carpentier KS; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA., Sanders W; Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Lucas CJ; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA., Jolly AJ; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA., Johnson CN; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA., Hawman DW; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA., Heise MT; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Moorman NJ; Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Morrison TE; Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2024 May 28; Vol. 16 (6). Date of Electronic Publication: 2024 May 28.
DOI: 10.3390/v16060861
Abstrakt: We previously reported that deletion of a 44-nucleotide element in the 3' untranslated region (UTR) of the Chikungunya virus (CHIKV) genome enhances the virulence of CHIKV infection in mice. Here, we find that while this 44-nucleotide deletion enhances CHIKV fitness in murine embryonic fibroblasts in a manner independent of the type I interferon response, the same mutation decreases viral fitness in C6/36 mosquito cells. Further, the fitness advantage conferred by the UTR deletion in mammalian cells is maintained in vivo in a mouse model of CHIKV dissemination. Finally, SHAPE-MaP analysis of the CHIKV 3' UTR revealed this 44-nucleotide element forms a distinctive two-stem-loop structure that is ablated in the mutant 3' UTR without altering additional 3' UTR RNA secondary structures.
Databáze: MEDLINE
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