High-Yield Production of Dihydroxyacetone from Crude Glycerol in Fed-Batch Cultures of Gluconobacter oxydans .

Autor: Górska K; Department of Biotechnology and Food Analysis, Wroclaw University of Economics and Business, 53-345 Wroclaw, Poland., Garncarek Z; Department of Biotechnology and Food Analysis, Wroclaw University of Economics and Business, 53-345 Wroclaw, Poland.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2024 Jun 20; Vol. 29 (12). Date of Electronic Publication: 2024 Jun 20.
DOI: 10.3390/molecules29122932
Abstrakt: The strain Gluconobacter oxydans LMG 1385 was used for the bioconversion of crude glycerol to dihydroxyacetone. The suitability of fed-batch cultures for the production of dihydroxyacetone was determined, and the influence of the pH of the culture medium and the initial concentration of glycerol on maximizing the concentration of dihydroxyacetone and on the yield and speed of obtaining dihydroxyacetone by bioconversion was examined. The feeding strategy of the substrate (crude glycerol) during the process was based on measuring the dissolved oxygen tension of the culture medium. The highest concentration of dihydroxyacetone P K = 175.8 g·L -1 and the highest yield Y P/Sw = 94.3% were obtained when the initial concentration of crude glycerol was S 0 = 70.0 g·L -1 and the pH of the substrate was maintained during the process at level 5.0.
Databáze: MEDLINE
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