| Autor: |
Alramadhan H; Department of Chemistry, College of Science, King Faisal University, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia., Elbashir AA; Department of Chemistry, College of Science, King Faisal University, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia.; Department of Chemistry, Faculty of Science, Khartoum University, P.O. Box 321, Khartoum 11114, Sudan., Alnajjar AO; Department of Chemistry, College of Science, King Faisal University, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia. |
| Jazyk: |
angličtina |
| Zdroj: |
Molecules (Basel, Switzerland) [Molecules] 2024 Jun 17; Vol. 29 (12). Date of Electronic Publication: 2024 Jun 17. |
| DOI: |
10.3390/molecules29122875 |
| Abstrakt: |
Atenolol (ATE) and propranolol (PRO) inclusion complexes with β-cyclodextrin have been investigated in aqueous solution. The aqueous solution was examined and characterized using UV-vis, fluorescence spectroscopy, and 1 H NMR. The physical mixture was characterized using FTIR. The existence of inclusion complexes is confirmed by observing changes in spectroscopic properties. The ATE complex with β-CD exhibited an interaction as host and (β-CD) as a guest in a 1:1 ratio, with an inclusion constant K of 2.09 × 10 -3 µM -1 , as determined by the typical double-reciprocal graphs. Similarly, the PRO complex with β-CD exhibited an interaction as host and (β-CD) guest in 1:1 and 1:2 stoichiometry at the same time; the inclusion constants were K1 = 5.80 × 10 -5 µM -1 and K2 = 4.67 × 10 -8 µM -1 , as determined by typical double-reciprocal graphs. The variables influencing the formation of the inclusion complexes were investigated and optimized. Based on the enhancement in fluorescence intensity due to the formation of inclusion complexes, spectrofluorometric methods were developed and validated for determination of each drug's pharmaceutical formulation. The quantification of the fluorescence intensity for ATE and PRO was conducted at λ ex /λ em 226/302 nm and λ ex /λ em 231/338 nm, respectively. Under the optimal reaction circumstances, linear relationships with good correlation coefficients of 0.9918 and 0.99 were found in the concentration ranges of 0.3-1.7 μM, and 0.1-1.1 μM for ATE and PRO, respectively. The limits of detection (LODs) were found to be 0.13 and 0.01 μM for ATE and PRO, respectively. The suggested approach was effectively applied to the analysis of both drugs' pharmaceutical formulations. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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