| Autor: |
Tran THT; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi 100000, Vietnam.; Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam., Hien BTT; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi 100000, Vietnam., Dung NTL; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi 100000, Vietnam., Huong NT; National Burn Hospital, Vietnam Military Medical University, Hanoi 100000, Vietnam., Binh TT; 103 Hospital, Vietnam Military Medical University, Hanoi 100000, Vietnam., Van Long N; Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi 100000, Vietnam., Ton ND; Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Institute of Genome Research, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam. |
| Abstrakt: |
Background and Objectives : Malaria continues to be a significant global health challenge. The efficacy of artemisinin-based combination therapies (ACTs) has declined in many parts of the Greater Mekong Subregion, including Vietnam, due to the spread of resistant malaria strains. This study was conducted to assess the efficacy of the Dihydroartemisinin (DHA)-Piperaquine (PPQ) regimen in treating uncomplicated falciparum malaria and to conduct molecular surveillance of antimalarial drug resistance in Binh Phuoc and Dak Nong provinces. Materials and Methods : The study included 63 uncomplicated malaria falciparum patients from therapeutic efficacy studies (TES) treated following the WHO treatment guidelines (2009). Molecular marker analysis was performed on all 63 patients. Methods encompassed Sanger sequencing for pfK13 mutations and quantitative real-time PCR for the pfpm2 gene. Results : This study found a marked decrease in the efficacy of the DHA-PPQ regimen, with an increased rate of treatment failures at two study sites. Genetic analysis revealed a significant presence of pfK13 mutations and pfpm2 amplifications, indicating emerging resistance to artemisinin and its partner drug. Conclusions : The effectiveness of the standard DHA-PPQ regimen has sharply declined, with rising treatment failure rates. This decline necessitates a review and possible revision of national malaria treatment guidelines. Importantly, molecular monitoring and clinical efficacy assessments together provide a robust framework for understanding and addressing detection drug resistance in malaria. |
