Enhancing Erucic Acid and Wax Ester Production in Brassica carinata through Metabolic Engineering for Industrial Applications.

Autor: Tesfaye M; Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 190, SE-234 22 Lomma, Sweden.; Institute of Biotechnology, Addis Ababa University, Addis Ababa P.O. Box. 1176, Ethiopia., Wang ES; Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 190, SE-234 22 Lomma, Sweden., Feyissa T; Institute of Biotechnology, Addis Ababa University, Addis Ababa P.O. Box. 1176, Ethiopia., Herrfurth C; Department of Plant Biochemistry, Albrecht Haller Institute for Plant Science, University of Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany.; Service Unit for Metabolomics and Lipidomics, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Justus-von-Liebig Weg 11, 37077 Goettingen, Germany., Haileselassie T; Institute of Biotechnology, Addis Ababa University, Addis Ababa P.O. Box. 1176, Ethiopia., Kanagarajan S; Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 190, SE-234 22 Lomma, Sweden., Feussner I; Department of Plant Biochemistry, Albrecht Haller Institute for Plant Science, University of Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany.; Department of Plant Biochemistry, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Justus-von-Liebig Weg 11, 37077 Goettingen, Germany., Zhu LH; Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 190, SE-234 22 Lomma, Sweden.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Jun 07; Vol. 25 (12). Date of Electronic Publication: 2024 Jun 07.
DOI: 10.3390/ijms25126322
Abstrakt: Metabolic engineering enables oilseed crops to be more competitive by having more attractive properties for oleochemical industrial applications. The aim of this study was to increase the erucic acid level and to produce wax ester (WE) in seed oil by genetic transformation to enhance the industrial applications of B. carinata . Six transgenic lines for high erucic acid and fifteen transgenic lines for wax esters were obtained. The integration of the target genes for high erucic acid ( BnFAE1 and LdPLAAT ) and for WEs ( ScWS and ScFAR ) in the genome of B. carinata cv. 'Derash' was confirmed by PCR analysis. The qRT-PCR results showed overexpression of BnFAE1 and LdPLAAT and downregulation of RNAi- BcFAD2 in the seeds of the transgenic lines. The fatty acid profile and WE content and profile in the seed oil of the transgenic lines and wild type grown in biotron were analyzed using gas chromatography and nanoelectrospray coupled with tandem mass spectrometry. A significant increase in erucic acid was observed in some transgenic lines ranging from 19% to 29% in relation to the wild type, with a level of erucic acid reaching up to 52.7%. Likewise, the transgenic lines harboring ScFAR and ScWS genes produced up to 25% WE content, and the most abundant WE species were 22:1/20:1 and 22:1/22:1. This study demonstrated that metabolic engineering is an effective biotechnological approach for developing B. carinata into an industrial crop.
Databáze: MEDLINE
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