Whole-Genome Analysis of Extensively Drug-Resistant Enterobacter hormaechei Isolated from a Patient with Non-Hodgkin's Lymphoma.
| Autor: | Ferreira CM; Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas-HEMOAM, Av. Constantino Nery, 4397, Chapada, Manaus 69050-001, Amazonas, Brazil., Naveca FG; Instituto Leônidas e Maria Deane-FIOCRUZ, Rua Teresina, 476, Adrianópolis, Manaus 69027-070, Amazonas, Brazil., Ferreira GMA; Programa de Pós-Graduação em Hematologia, Universidade do Estado do Amazonas-PPGH-UEA/HEMOAM, Av. Constantino Nery, 4397, Chapada, Manaus 69050-001, Amazonas, Brazil., Barbosa MNS; Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas-HEMOAM, Av. Constantino Nery, 4397, Chapada, Manaus 69050-001, Amazonas, Brazil., de Souza VC; Instituto Leônidas e Maria Deane-FIOCRUZ, Rua Teresina, 476, Adrianópolis, Manaus 69027-070, Amazonas, Brazil., Calheiros FO; Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas-HEMOAM, Av. Constantino Nery, 4397, Chapada, Manaus 69050-001, Amazonas, Brazil., Souza VS; Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas-HEMOAM, Av. Constantino Nery, 4397, Chapada, Manaus 69050-001, Amazonas, Brazil., Ferreira WA; Fundação de Dermatologia Tropical e Venereologia Alfredo da Matta-FUAM, Rua Codajás, 24, Cachoeirinha, Manaus 69065-130, Amazonas, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Genes [Genes (Basel)] 2024 Jun 20; Vol. 15 (6). Date of Electronic Publication: 2024 Jun 20. |
| DOI: | 10.3390/genes15060814 |
| Abstrakt: | Background: Currently, the Enterobacteriaceae species are responsible for a variety of serious infections and are already considered a global public health problem, especially in underdeveloped countries, where surveillance and monitoring programs are still scarce and limited. Analyses were performed on the complete genome of an extensively antibiotic-resistant strain of Enterobater hormaechei , which was isolated from a patient with non-Hodgkin's lymphoma, who had been admitted to a hospital in the city of Manaus, Brazil. Methods: Phenotypical identification and susceptibility tests were performed in automated equipment. Total DNA extraction was performed using the PureLink genomic DNA mini-Kit. The genomic DNA library was prepared with Illumina Microbial Amplicon Prep and sequenced in the MiSeq Illumina Platform. The assembly of the whole-genome and individual analyses of specific resistance genes extracted were carried out using online tools and the Geneious Prime software. Results: The analyses identified an extensively resistant ST90 clone of E. hormaechei carrying different genes, including bla Conclusions: These findings highlight the need for actions to control and monitor these pathogens in the hospital environment. |
| Databáze: | MEDLINE |
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