| Autor: |
De Iorio MG; Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, Italy., Lazzari B; Institute of Agricultural Biology and Biotechnology, Consiglio Nazionale delle Ricerche, 20133 Milano, Italy., Colli L; Department of Animal, Nutrition and Food Sciences and Research Center on Biodiversity and Ancient DNA, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy., Pagnacco G; Institute of Agricultural Biology and Biotechnology, Consiglio Nazionale delle Ricerche, 20133 Milano, Italy., Minozzi G; Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, Italy. |
| Abstrakt: |
In Apis mellifera , csd is the primary gene involved in sex determination: haploid hemizygous eggs develop as drones, while females develop from eggs heterozygous for the csd gene. If diploid eggs are homozygous for the csd gene, diploid drones will develop, but will be eaten by worker bees before they are born. Therefore, high csd allelic diversity is a priority for colony survival and breeding. This study aims to investigate the variability of the hypervariable region (HVR) of the csd gene in bees sampled in an apiary under a selection scheme. To this end, an existing dataset of 100 whole-genome sequences was analyzed with a validated pipeline based on de novo assembly of sequences within the HVR region. In total, 102 allelic sequences were reconstructed and translated into amino acid sequences. Among these, 47 different alleles were identified, 44 of which had previously been observed, while 3 are novel alleles. The results show a high variability in the csd region in this breeding population of honeybees. |
