Modulation of adipose-derived stem cell behavior by prostate pathology-associated plasma: insights from in vitro exposure.

Autor: Cruciani S; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy., Coradduzza D; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy., Balzano F; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy., Garroni G; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy., Azara E; Institute of Biomolecular Chemistry, National Research Council, 07100, Sassari, Italy., Pala R; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy., Delitala AP; Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100, Sassari, Italy., Madonia M; Department of Clinical and Experimental Medicine, Urologic Clinic, University of Sassari, Sassari, Italy., Tedde A; Department of Clinical and Experimental Medicine, Urologic Clinic, University of Sassari, Sassari, Italy., Capobianco G; Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100, Sassari, Italy., Petrillo M; Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100, Sassari, Italy., Angelucci C; Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100, Sassari, Italy., Carru C; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy.; Medical Oncology Unit, University Hospital (AOU) of Sassari, 07100, Sassari, Italy., Ventura C; Laboratory of Molecular Biology and Stem Cell Engineering, Istituto Nazionale Biostrutture E Biosistemi (INBB)-Eldor Lab, Via Corticella 183, 40128, Bologna, Italy. ventura.vid@gmail.com., Maioli M; Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy. mmaioli@uniss.it.; Center for Developmental Biology and Reprogramming-CEDEBIOR, Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy. mmaioli@uniss.it.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jun 26; Vol. 14 (1), pp. 14765. Date of Electronic Publication: 2024 Jun 26.
DOI: 10.1038/s41598-024-64625-0
Abstrakt: Adipose-derived stem cells (ADSCs) are promising in regenerative medicine. Their proliferation, survival and activation are influenced by specific signals within their microenvironment, also known as niche. The stem cell niche is regulated by complex interactions between multiple cell types. When transplanted in a specific area, ADSCs can secrete several immunomodulatory factors. At the same time, a tumor microenvironment can influence stem cell behavior, modulating proliferation and their ability to differentiate into a specific phenotype. Whitin this context, we exposed ADSCs to plasma samples derived from human patients diagnosed with prostate cancer (PC), or precancerous lesions (PL), or benign prostatic hyperplasia (BPH) for 4, 7 or 10 days. We then analyzed the expression of main stemness-related markers and cell-cycle regulators. We also measured cytokine production and polyamine secretion in culture medium and evaluated cell morphology and collagen production by confocal microscopy. The results obtained from this study show significant changes in the morphology of ADSCs exposed to plasma samples, especially in the presence of prostate cancer plasma, suggesting important implications in the use of ADSCs for the development of new treatments and application in regenerative medicine.
(© 2024. The Author(s).)
Databáze: MEDLINE
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