Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics in Bacillus subtilis.

Autor: Middlemiss S; Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK. stuart.middlemiss@newcastle.ac.uk., Blandenet M; Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK., Roberts DM; School of Life Sciences, University of Warwick, Gibbet Hill Campus, Coventry, UK., McMahon A; School of Life Sciences, University of Warwick, Gibbet Hill Campus, Coventry, UK., Grimshaw J; Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK., Edwards JM; Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.; School of Life Sciences, University of Warwick, Gibbet Hill Campus, Coventry, UK., Sun Z; Department of Electronic Engineering, The Chinese University of Hong Kong, Hong Kong, China., Whitley KD; Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK., Blu T; Department of Electronic Engineering, The Chinese University of Hong Kong, Hong Kong, China.; Dept of Electrical Engineering, National Taiwan University, Taipei City, Taiwan., Strahl H; Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK. h.strahl@newcastle.ac.uk., Holden S; Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK. seamus.holden@warwick.ac.uk.; School of Life Sciences, University of Warwick, Gibbet Hill Campus, Coventry, UK. seamus.holden@warwick.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Jun 26; Vol. 15 (1), pp. 5411. Date of Electronic Publication: 2024 Jun 26.
DOI: 10.1038/s41467-024-49785-x
Abstrakt: Most rod-shaped bacteria elongate by inserting new cell wall material into the inner surface of the cell sidewall. This is performed by class A penicillin binding proteins (PBPs) and a highly conserved protein complex, the elongasome, which moves processively around the cell circumference and inserts long glycan strands that act as barrel-hoop-like reinforcing structures, thereby giving rise to a rod-shaped cell. However, it remains unclear how elongasome synthesis dynamics and termination events are regulated to determine the length of these critical cell-reinforcing structures. To address this, we developed a method to track individual elongasome complexes around the entire circumference of Bacillus subtilis cells for minutes-long periods using single-molecule fluorescence microscopy. We found that the B. subtilis elongasome is highly processive and that processive synthesis events are frequently terminated by rapid reversal or extended pauses. We found that cellular levels of RodA regulate elongasome processivity, reversal and pausing. Our single-molecule data, together with stochastic simulations, show that elongasome dynamics and processivity are regulated by molecular motor tug-of-war competition between several, likely two, oppositely oriented peptidoglycan synthesis complexes associated with the MreB filament. Altogether these results demonstrate that molecular motor tug-of-war is a key regulator of elongasome dynamics in B. subtilis, which likely also regulates the cell shape via modulation of elongasome processivity.
(© 2024. The Author(s).)
Databáze: MEDLINE
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