Proteomic strategies to interrogate the Fe-S proteome.

Autor: Bak DW; Department of Chemistry, Boston College, Chestnut Hill, MA, United States of America. Electronic address: bakd@bc.edu., Weerapana E; Department of Chemistry, Boston College, Chestnut Hill, MA, United States of America. Electronic address: eranthie@bc.edu.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Molecular cell research [Biochim Biophys Acta Mol Cell Res] 2024 Oct; Vol. 1871 (7), pp. 119791. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1016/j.bbamcr.2024.119791
Abstrakt: Iron‑sulfur (Fe-S) clusters, inorganic cofactors composed of iron and sulfide, participate in numerous essential redox, non-redox, structural, and regulatory biological processes within the cell. Though structurally and functionally diverse, the list of all proteins in an organism capable of binding one or more Fe-S clusters is referred to as its Fe-S proteome. Importantly, the Fe-S proteome is highly dynamic, with continuous cluster synthesis and delivery by complex Fe-S cluster biogenesis pathways. This cluster delivery is balanced out by processes that can result in loss of Fe-S cluster binding, such as redox state changes, iron availability, and oxygen sensitivity. Despite continued expansion of the Fe-S protein catalogue, it remains a challenge to reliably identify novel Fe-S proteins. As such, high-throughput techniques that can report on native Fe-S cluster binding are required to both identify new Fe-S proteins, as well as characterize the in vivo dynamics of Fe-S cluster binding. Due to the recent rapid growth in mass spectrometry, proteomics, and chemical biology, there has been a host of techniques developed that are applicable to the study of native Fe-S proteins. This review will detail both the current understanding of the Fe-S proteome and Fe-S cluster biology as well as describing state-of-the-art proteomic strategies for the study of Fe-S clusters within the context of a native proteome.
Competing Interests: Declaration of competing interest The authors declare no competing financial or personal interests that could have appeared to influence the work reported in this manuscript.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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