Hormonal dependent expression of apelin and apelin receptor in the ovary and uterus of mice.

Autor: Anima B; Department of Zoology, Mizoram University, Aizawl 796004, Mizoram, India., Gurusubramanian G; Department of Zoology, Mizoram University, Aizawl 796004, Mizoram, India., Roy VK; Department of Zoology, Mizoram University, Aizawl 796004, Mizoram, India. Electronic address: vikasroy4araria@yahoo.co.in.
Jazyk: angličtina
Zdroj: Reproductive biology [Reprod Biol] 2024 Sep; Vol. 24 (3), pp. 100918. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1016/j.repbio.2024.100918
Abstrakt: Apelin and APJ have been shown to regulate female reproductive functions. However, its uterine expression during the oestrous cycle and its regulation by ovarian steroids, along with gonadotropin regulation in the ovary, has not been investigated. This study aimed to analyze the steroid-dependent uterine expression of apelin/APJ in the uterus along with the oestrous cycle. Furthermore, it also aimed to investigate gonadotropin-dependent ovarian expression of apelin and APJ. To investigate the uterine expression of apelin and APJ during estrous cycle in mice, uterus at different estrous stage were collected. To explore the ovarian steroids dependent expression of apelin system in the uterus, ovariectomized mice were treated with only estrogen at dose of 30 ng/g, only progesterone at dose of 150 μg/g and combined doses. To study the effect of gonadotropin on ovarian expression of apelin system, immature mice were injected with 2.5 IU of pregnant mare serum gonadotropin (PMSG) alone and both PMSG plus 2.5 IU of chorionic gonadotropin (hCG). Apelin and APJ protein expression are modulated by estrous phases in the uterus. The uterine apelin and APJ expression are up-regulated by estrogen and down-regulated by progesterone. The expression and localization of APJ showed increased abundance in the follicles of PMSG treated mice, however, the PMSG plus HCG treatment showed formation of corpus luteum with increased abundance of APJ and progesterone secretion. The expression of apelin and APJ are regulated by pituitary gonadotropin in the ovary and uterine apelin system by ovarian steroid hormone.
Competing Interests: Declaration of Competing Interest All authors declared that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
(Copyright © 2024 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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