Development of capillary electrophoresis methods for the detection of microbial metabolites on potential future spaceflight missions.

Autor: Kurfman EA; Department of Chemistry, University of Kansas, Lawrence, Kansas, USA.; Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, Kansas, USA., Mora MF; Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California, USA., Willis PA; Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California, USA., Lunte SM; Department of Chemistry, University of Kansas, Lawrence, Kansas, USA.; Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, Kansas, USA.; Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas, USA.
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2024 Oct; Vol. 45 (19-20), pp. 1684-1691. Date of Electronic Publication: 2024 Jun 26.
DOI: 10.1002/elps.202400073
Abstrakt: The search for chemical indicators of life is a fundamental component of potential future spaceflight missions to ocean worlds. Capillary electrophoresis (CE) is a useful separation method for the determination of the small organic molecules, such as amino acids and nucleobases, that could be used to help determine whether or not life is present in a sample collected during such missions. CE is under development for spaceflight applications using multiple detection systems, such as laser induced fluorescence (LIF) and mass spectrometry (MS). Here we report CE-based methods for separation and detection of major polar metabolites in cells, such as amino acids, nucleobases/sides, and oxidized and reduced glutathione using detectors that are less expensive alternatives to LIF and MS. Direct UV detection, indirect UV detection, and capacitvely coupled contactless conductivity detection (C 4 D) were tested with CE, and a combination of direct UV and C 4 D allowed the detection of the widest variety of metabolites. The optimized method was used to profile metabolites found in samples of Escherichia coli and Pseudoalteromonas haloplanktis and showed distinct differences between the species.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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