Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors.

Autor:	Kourelis J; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK.; The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Norwich, UK., Schuster M; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., Demir F; Central Institute for Engineering, Department of Electronics and Analytics (ZEA), Analytics (ZEA-3), Research Centre Jülich, Wilhelm-Johnen-Str., 52428 Jülich, Germany., Mattinson O; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., Krauter S; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., Kahlon PS; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., O'Grady R; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., Royston S; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., Bravo-Cazar AL; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., Mooney BC; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK., Huesgen PF; Central Institute for Engineering, Department of Electronics and Analytics (ZEA), Analytics (ZEA-3), Research Centre Jülich, Wilhelm-Johnen-Str., 52428 Jülich, Germany., Kamoun S; The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Norwich, UK., van der Hoorn RAL; The Plant Chemetics Laboratory, Department of Biology, University of Oxford, South Parks Road, OX1 3RB Oxford, UK.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Sep 03; Vol. 36 (9), pp. 3260-3276.
DOI:	10.1093/plcell/koae183
Abstrakt:	Secreted immune proteases "Required for Cladosporium resistance-3" (Rcr3) and "Phytophthora-inhibited protease-1" (Pip1) of tomato (Solanum lycopersicum) are both inhibited by Avirulence-2 (Avr2) from the fungal plant pathogen Cladosporium fulvum. However, only Rcr3 acts as a decoy co-receptor that detects Avr2 in the presence of the Cf-2 immune receptor. Here, we identified crucial residues in tomato Rcr3 that are required for Cf-2-mediated signaling and bioengineered various proteases to trigger Avr2/Cf-2-dependent immunity. Despite substantial divergence in Rcr3 orthologs from eggplant (Solanum melongena) and tobacco (Nicotiana spp.), minimal alterations were sufficient to trigger Avr2/Cf-2-mediated immune signaling. By contrast, tomato Pip1 was bioengineered with 16 Rcr3-specific residues to initiate Avr2/Cf-2-triggered immune signaling. These residues cluster on one side of the protein next to the substrate-binding groove, indicating a potential Cf-2 interaction site. Our findings also revealed that Rcr3 and Pip1 have distinct substrate preferences determined by two variant residues and that both are suboptimal for binding Avr2. This study advances our understanding of Avr2 perception and opens avenues to bioengineer proteases to broaden pathogen recognition in other crops. Competing Interests: Conflict of interest statement: None declared, except for J.K., whom received funding from industry during part of this study. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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