Live and Dead Clostridium butyricum GKB7 Diminish Osteoarthritis Pain and Progression in Preclinical Animal Model.

Autor: Chen LC; Department of Pharmacy, Tajen University, Pingtung, Taiwan., Lin YY; Department of Pharmacology, School of Medicine, China Medical University, Taichung, Taiwan., Tsai YS; Biotech Research Institute, Grape King Bio Ltd., Taoyuan, Taiwan., Chen CC; Biotech Research Institute, Grape King Bio Ltd., Taoyuan, Taiwan.; Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan., Chang TC; Department of Pharmacology, School of Medicine, China Medical University, Taichung, Taiwan., Chen HT; Department of Sports Medicine, College of Health Care, China Medical University, Taichung, Taiwan.; Department of Orthopedic Surgery, China Medical University Hospital, Taichung, Taiwan., Hsu CJ; Department of Orthopedic Surgery, China Medical University Hospital, Taichung, Taiwan.; School of Chinese Medicine, China Medical University, Taichung, Taiwan., Tang CH; Department of Pharmacology, School of Medicine, China Medical University, Taichung, Taiwan.; Chinese Medicine Research Center, China Medical University, Taichung, Taiwan.; Department of Medical Laboratory Science and Biotechnology, College of Medical and Health Science, Asia University, Taichung, Taiwan.; Department of Medical Research, China Medical University Hsinchu Hospital, Hsinchu, Taiwan.
Jazyk: angličtina
Zdroj: Environmental toxicology [Environ Toxicol] 2024 Nov; Vol. 39 (11), pp. 4927-4935. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1002/tox.24367
Abstrakt: Osteoarthritis (OA) is a degenerative joint disease primarily affecting the elderly. It is characterized by the progressive decline of joint cartilage and alterations in the underlying bone. Several probiotic strains have exhibited immunomodulatory and anti-inflammatory properties. Here, we examined the functions of live and dead Clostridium butyricum GKB7 (GKB7-L and GKB7-D) in a preclinical anterior cruciate ligament transection (ACLT)-enhanced OA procedure. Oral administration of GKB7-L and GKB7-D ameliorated ACLT-induced bone pain as assessed by weight-bearing behavioral testing but did not affect body weight. Micro-computed tomography (CT) results showed that GKB7-L and GKB7-D diminished ACLT-induced bone destruction and loss. GKB7-L and GKB7-D-enriched therapies also reduced ACLT-induced production of the pro-inflammatory cytokines interleukin (IL)-1β and tumor necrosis factor (TNF)-α, as well as the chondrolytic factor matrix metalloproteinase (MMP)-3, leading to inhibition of aggrecan and collagen type II degradation and thereby blocking cartilage breakdown. We therefore suggest that oral supplementation with GKB7-L or GKB7-D can be beneficial in the prevention and treatment of OA.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: