Single-cell proteomics differentiates Arabidopsis root cell types.

Autor: Montes C; Department of Plant Pathology, Entomology, and Microbiology, Iowa State University, Ames, IA, 50011, USA., Zhang J; Department of Biology, Duke University, Durham, NC, 27708, USA., Nolan TM; Department of Biology, Duke University, Durham, NC, 27708, USA.; Howard Hughes Medical Institute, Duke University, Durham, NC, 27708, USA., Walley JW; Department of Plant Pathology, Entomology, and Microbiology, Iowa State University, Ames, IA, 50011, USA.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Dec; Vol. 244 (5), pp. 1750-1759. Date of Electronic Publication: 2024 Jun 24.
DOI: 10.1111/nph.19923
Abstrakt: Single-cell proteomics (SCP) is an emerging approach to resolve cellular heterogeneity within complex tissues of multi-cellular organisms. Here, we demonstrate the feasibility of SCP on plant samples using the model plant Arabidopsis thaliana. Specifically, we focused on examining isolated single cells from the cortex and endodermis, which are two adjacent root cell types derived from a common stem cell lineage. From 756 root cells, we identified 3763 proteins and 1118 proteins/cell. Ultimately, we focus on 3217 proteins quantified following stringent filtering. Of these, we identified 596 proteins whose expression is enriched in either the cortex or endodermis and are able to differentiate these closely related plant cell types. Collectivity, this study demonstrates that SCP can resolve neighboring cell types with distinct functions, thereby facilitating the identification of biomarkers and candidate proteins to enable functional genomics.
(© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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