High Sensitivity Stimulated Raman Scattering Microscopy with Electronic Resonance Enhancement.

Autor: Pruccoli A; Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78464, Konstanz, Germany., Zumbusch A; Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78464, Konstanz, Germany.
Jazyk: angličtina
Zdroj: Chemphyschem : a European journal of chemical physics and physical chemistry [Chemphyschem] 2024 Oct 01; Vol. 25 (19), pp. e202400309. Date of Electronic Publication: 2024 Sep 09.
DOI: 10.1002/cphc.202400309
Abstrakt: Raman microscopy is an important tool for labelfree microscopy. However, spontaneous Raman microscopy suffers from slow image acquisition rates and susceptibility to fluorescence background. Coherent Raman microsocopy techniques such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS) microscopy, by contrast, offer fast imaging capability and robustness against sample fluorescence. Yet, their rather low sensitivity impedes their broader application. This review discusses sensitivity enhancement of SRS microscopy to μ ${\mu }$ M detection levels by using electronically pre-resonant excitation. We present the foundations of this approach, discuss its technological implementation, and show first successful applications. A special emphasis is given to outlining new experimental developments allowing novel types of investigations.
(© 2024 The Authors. ChemPhysChem published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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