Biosynthesis of gold nanoparticles by fungi and its potential in SERS.

Autor: Olvera-Aripez J; Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, B.C, Mexico., Camacho-López S; Optics Department, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, B.C, Mexico., Flores-Castañeda M; Optics Department, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, B.C, Mexico., Belman-Rodríguez C; Center for Nanosciences and Nanotechnology (CNyN), National Autonomous University of Mexico (UNAM), Ensenada, B.C, Mexico., Vilchis-Nestor AR; Sustainable Chemistry Research Joint Center UAEM-UNAM (CCIQS), Carr. Toluca-Atlacomulco km 14.5, San Cayetano, 50200, Toluca, Mexico., Castro-Longoria E; Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE), Ensenada, B.C, Mexico. ecastro@cicese.mx.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2024 Sep; Vol. 47 (9), pp. 1585-1593. Date of Electronic Publication: 2024 Jun 26.
DOI: 10.1007/s00449-024-03053-w
Abstrakt: Surface enhanced Raman spectroscopy (SERS) by using gold nanoparticles (AuNPs) has gained relevance for the identification of biomolecules and some cancer cells. Searching for greener NPs synthesis alternatives, we evaluated the SERS properties of AuNPs produced by using different filamentous fungi. The AuNPs were synthesized utilizing the supernatant of Botrytis cinerea, Trichoderma atroviride, Trichoderma asperellum, Alternaria sp. and Ganoderma sessile. The AuNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis) to identify its characteristic surface plasmon resonance, which was located at 545 nm (B. cinerea), 550 nm (T. atroviride), 540 nm (T. asperellum), 530 nm (Alternaria sp.), and 525 nm (G. sessile). Morphology, size and crystal structure were characterized through transmission electron microscopy (TEM); colloidal stability was assessed by Z-potential measurements. We found that, under specific incubation conditions, it was possible to obtain AuNPs with spherical and quasi-spherical shapes, which mean size range depends on the fungal species supernatant with 92.9 nm (B. cinerea), 24.7 nm (T. atroviride), 16.4 nm (T. asperellum), 9.5 nm (Alternaria sp.), and 13.6 nm (G. sessile). This, as it can be expected, has an effect on Raman amplification. A micro-Raman spectroscopy system operated at a wavelength of 532 nm was used for the evaluation of the SERS features of the AuNPs. We chose methylene blue as our target molecule since it has been widely used for such a purpose in the literature. Our results show that AuNPs synthesized with the supernatant of T. atroviride, T. asperellum and Alternaria sp. produce the stronger SERS effect, with enhancement factor (EF) of 20.9, 28.8 and 35.46, respectively. These results are promising and could serve as the base line for the development of biosensors through a facile, simple, and low-cost green alternative.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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