| Autor: |
Titon MC; Programa de Pós-Graduação em Produção e Sanidade Animal, Instituto Federal Catarinense - IFC, Campus Concordia, Rodovia SC 283, km 17, s/n, 89703-720 Concórdia, SC, Brazil., Dezen D; Programa de Pós-Graduação em Produção e Sanidade Animal, Instituto Federal Catarinense - IFC, Campus Concordia, Rodovia SC 283, km 17, s/n, 89703-720 Concórdia, SC, Brazil., Costa PMA; Programa de Pós-Graduação em Produção e Sanidade Animal, Instituto Federal Catarinense - IFC, Campus Concordia, Rodovia SC 283, km 17, s/n, 89703-720 Concórdia, SC, Brazil., Rizzotto DW; Universidade do Oeste de Santa Catarina - UNOESC, Rua Dirceu Giordani, 696, Jardim Tarumã, 89820-000 Xanxerê, SC, Brazil., Castanha SF; Universidade do Oeste de Santa Catarina - UNOESC, Rua Dirceu Giordani, 696, Jardim Tarumã, 89820-000 Xanxerê, SC, Brazil., Millezi AF; Programa de Pós-Graduação em Produção e Sanidade Animal, Instituto Federal Catarinense - IFC, Campus Concordia, Rodovia SC 283, km 17, s/n, 89703-720 Concórdia, SC, Brazil. |
| Abstrakt: |
Detection of Salmonella sp. is important for the broiler chicken production chain because it is one microorganisms involved in food-borne diseases. Thus, this study performed the optimization of a technique of Loop-mediated isothermal DNA amplification (LAMP) through the 3MTM Molecular Detection Assay 2: Salmonella (MDS®), in accordance with Ordinance number 126 of the Ministry of Agriculture, for the detection of Salmonella sp. in drag swab. The methodology followed ISO 16140-2: 2016, with the analysis naturally contaminated drag swab samples collected from broiler aviaries and artificially contaminated with salmonella ATCCs. Of the 300 samples processed in protocol A (pre-enrichment tetrathionate broth (TT)), 45 were positive for Salmonella sp., 242 negative, one false-positive, and 12 false-negative, while of the 300 samples analyzed in protocol B (pre-enrichment brain-heart infusion broth (BHI)), 40 were positive, 256 negative, one false-positive, and three false-negative. The result for protocol A was a sensitivity of 79%, specificity of 99.6%, Positive Predictive Value (PPV) of 98%, and Negative Predictive Value (NPV) of 95%; and for protocol B, 93% sensitivity, 99.6% specificity, 98% PPV, and 99% NPV. Both protocols were associated with the reference method (p>0.05), concluding that the MDS® can be used for the qualitative detection of Salmonella sp. |
