| Autor: |
Mooney R; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., Giammarini E; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., Corbett E; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., Thomson S; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., McKinley K; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., Sinisterra Sebastian P; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., Rodgers K; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., O'Donnell J; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK., McGinness C; School of Computing, Engineering and Physical Sciences, University of the West of Scotland Paisley, Glasgow PA1 2BE, UK., Roberts CW; Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UK., Ramaesh K; NHS Greater Glasgow and Clyde, Gartnavel Hospital, Department of Ophthalmology, Glasgow G12 0YN, UK., Henriquez FL; School of Health and Life Sciences, University of the West of Scotland Lanarkshire, Glasgow G72 0LH, UK. |
| Abstrakt: |
Acanthamoeba keratitis (AK) is a severe infection of the cornea. Prevention and treatment are difficult due to the inefficacy of currently available compounds. The impact of many commonly used compounds for routine examinations of Acanthamoeba is unexplored but might offer insight useful in combatting AK. In this study, we demonstrate that sodium metabisulfite, a common preservation constituent of eye care solutions, was found to be active against Acanthamoeba trophozoites at concentrations lower than that commonly found in eye drops (IC 50 0.03 mg/mL). We demonstrate that sodium metabisulfite depletes thiamine from growth medium and that Acanthamoeba is a thiamine auxotroph, requiring thiamine salvage for growth. The inhibitory effects of sodium metabisulfite can be overcome by thiamine supplementation. These results are consistent with the lack of key enzymes for thiamine biosynthesis in the genome of Acanthamoeba , an area which might prove exploitable using new or existing compounds. Indeed, this study highlights sodium metabisulfite as a useful inhibitor of Acanthamoeba castellanii trophozoites in vitro and that it acts, at least in part, by limiting available thiamine. |
