| Autor: |
Maniam N; Pharmacognosy Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang Darul Makmur, Malaysia., Khatib A; Pharmacognosy Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang Darul Makmur, Malaysia.; Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia., Ahmed QU; Pharmacognosy Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang Darul Makmur, Malaysia., Ibrahim Z; Pharmacognosy Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang Darul Makmur, Malaysia., Syed Mohamad SNA; Pharmacognosy Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang Darul Makmur, Malaysia., Nipun TS; Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong, Bangladesh., Humaryanto; Faculty of Medicine, Universitas Jambi, Jambi, Indonesia. |
| Abstrakt: |
Metabolite profiling is required to reveal bioactive chemicals in ginger rhizome for supporting its traditional claim as anti-diabetic agent. This study aimed to evaluate α-glucosidase inhibitory (AGI) and antioxidant activities of the rhizome, to identify its putative α-glucosidase inhibitors, and to analyse the protein-ligand interaction of the inhibitors. The ginger extracts were tested to in vitro AGI assay and analysed using LCMS-based metabolomics to pinpoint the putative α-glucosidase inhibitors. The methanol extract exhibited the highest AGI activity (IC 50 = 185.2 µg/mL) compared to the other extracts. This extract showed antioxidant activities with DPPH-IC 50 and FRAP value of 125.0 µg/mL and 16.95 mmol TE/mgDW, respectively. The LCMS-based metabolomics revealed α-glucosidase inhibitors in the extract, namely 7-methoxycoumarin, supinine and 12-hydroxycorynoline. The presence of these compounds in ginger is being reported for the first time in this study. The activity of these compounds was supported by computational study using in silico molecular docking. |
