Recovery of Proteases and Protease Inhibitors from Ganoderma spp. Cultivated in Amazonian Lignocellulose Wastes.

Autor:	Ramos Chevreuil L; Edible Fungi Cultivation Laboratory, National Institute for Amazonian Research, Av. André Araújo 69067-375, Amazonas, Brazil., Alves Pessoa V; Edible Fungi Cultivation Laboratory, National Institute for Amazonian Research, Av. André Araújo 69067-375, Amazonas, Brazil.; Postgraduate Program in Biotechnology, Federal University of Amazonas, Av. General Rodrigo Octavio69067-005, Amazonas, Brazil., Lima da Silva G; Edible Fungi Cultivation Laboratory, National Institute for Amazonian Research, Av. André Araújo 69067-375, Amazonas, Brazil.; Postgraduate Program in Biotechnology, Federal University of Amazonas, Av. General Rodrigo Octavio69067-005, Amazonas, Brazil., Dos Santos Gouvêa PR; Edible Fungi Cultivation Laboratory, National Institute for Amazonian Research, Av. André Araújo 69067-375, Amazonas, Brazil.; Postgraduate Program in Biotechnology, Federal University of Amazonas, Av. General Rodrigo Octavio69067-005, Amazonas, Brazil., Batista do Nascimento Soares L; Edible Fungi Cultivation Laboratory, National Institute for Amazonian Research, Av. André Araújo 69067-375, Amazonas, Brazil.; Postgraduate Program in Biodiversity and Biotechnology of the Bionorte Network, State University of Amazonas, Av. Carvalho Leal69065-001, Amazonas, Brazil., Sales-Campos C; Edible Fungi Cultivation Laboratory, National Institute for Amazonian Research, Av. André Araújo 69067-375, Amazonas, Brazil.; Postgraduate Program in Biotechnology, Federal University of Amazonas, Av. General Rodrigo Octavio69067-005, Amazonas, Brazil.; Postgraduate Program in Biodiversity and Biotechnology of the Bionorte Network, State University of Amazonas, Av. Carvalho Leal69065-001, Amazonas, Brazil.
Jazyk:	angličtina
Zdroj:	Current protein & peptide science [Curr Protein Pept Sci] 2024 Jun 24. Date of Electronic Publication: 2024 Jun 24.
DOI:	10.2174/0113892037297181240605112831
Abstrakt:	Background: Ganoderma spp. are a great source of bioactive molecules. The production and recovery of bioactive molecules vary according to strain, growth substrate, and extraction solution. Variations in protease and their inhibitors in basidiomata from a commercial strain (G. lingzhi) and an Amazonian isolate (Ganoderma sp.) cultivated in Amazonian lignocellulosic wastes and extracted with different solutions are plausible and were investigated in our study. Methods: Basidiomata from cultivation in substrates based on açaí seed, guaruba-cedro sawdust and three lots of marupá sawdust were submitted to extraction in water, Tris-HCl, and sodium phosphate. Protein content, proteases, and protease inhibitors were estimated through different assays. The samples were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR). Results: Tris-HCl provided higher protein extraction from Ganoderma sp. and higher caseinolytic, gelatinolytic, and fibrinolytic activity for G. lingzhi cultivated in açaí. Water extracts of Ganoderma sp., in general, exhibited higher trypsin and papain inhibitor activities compared to G. lingzhi. Extracts in Tris-HCl and sodium phosphate showed more intense protein bands in SDS-- PAGE, highlighting bands of molecular weights around 100, 50, and 30 kDa. FTIR spectra showed patterns for proteins in all extracts, with variation in transmittance according to substrate and extractor. Conclusion: Water extract from Amazonian Ganoderma sp. cultivated in marupá wastes are promising as a source of protease inhibitors, while the Tris-HCL extract of G. lingzhi from açaí cultivation stands out as a source of proteases with fibrinolytic, caseinolytic, and gelatinolytic activities. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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