Harnessing extracellular vesicles-mediated signaling for enhanced bone regeneration: novel insights into scaffold design.

Autor:	Kanniyappan H; Regeneratve Medicine and Disability Research Laboratory (RMDR), Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL, United States of America.; Department of Chemistry, Illinois Institute of Technology (IIT), Chicago, IL, United States of America., Gnanasekar V; University of Wisconsin-Madison, Madison, WI, United States of America., Parise V; Regeneratve Medicine and Disability Research Laboratory (RMDR), Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL, United States of America., Debnath K; College of Dentistry, University of Illinois, Chicago, IL, United States of America., Sun Y; Department of Material Sciences, University of Illinois, Chicago, IL, United States of America., Thakur S; Regeneratve Medicine and Disability Research Laboratory (RMDR), Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL, United States of America., Thakur G; Regeneratve Medicine and Disability Research Laboratory (RMDR), Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL, United States of America., Perumal G; Regeneratve Medicine and Disability Research Laboratory (RMDR), Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL, United States of America., Kumar R; Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, India., Wang R; Department of Chemistry, Illinois Institute of Technology (IIT), Chicago, IL, United States of America., Merchant A; Regeneratve Medicine and Disability Research Laboratory (RMDR), Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL, United States of America., Sriram R; College of Dentistry, University of Illinois, Chicago, IL, United States of America., Mathew MT; Regeneratve Medicine and Disability Research Laboratory (RMDR), Department of Biomedical Sciences, University of Illinois College of Medicine, Rockford, IL, United States of America.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Jul 04; Vol. 19 (5). Date of Electronic Publication: 2024 Jul 04.
DOI:	10.1088/1748-605X/ad5ba9
Abstrakt:	The increasing prevalence of bone replacements and complications associated with bone replacement procedures underscores the need for innovative tissue restoration approaches. Existing synthetic grafts cannot fully replicate bone vascularization and mechanical characteristics. This study introduces a novel strategy utilizing pectin, chitosan, and polyvinyl alcohol to create interpenetrating polymeric network (IPN) scaffolds incorporated with extracellular vesicles (EVs) isolated from human mesenchymal stem cells (hMSCs). We assess the osteointegration and osteoconduction abilities of these models in vitro using hMSCs and MG-63 osteosarcoma cells. Additionally, we confirm exosome properties through Transmission Electron Microscopy (TEM), immunoblotting, and Dynamic Light Scattering (DLS). In vivo , chick allantoic membrane assay investigates vascularization characteristics. The study did not include in vivo animal experiments. Our results demonstrate that the IPN scaffold is highly porous and interconnected, potentially suitable for bone implants. EVs, approximately 100 nm in size, enhance cell survival, proliferation, alkaline phosphatase activity, and the expression of osteogenic genes. EVs-mediated IPN scaffolds demonstrate promise as precise drug carriers, enabling customized treatments for bone-related conditions and regeneration efforts. Therefore, the EVs-mediated IPN scaffolds demonstrate promise as precise carriers for the transport of drugs, allowing for customized treatments for conditions connected to bone and efforts in regeneration. (© 2024 IOP Publishing Ltd.)
Databáze:	MEDLINE
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