Effect of bile salts on intestinal epithelial function in gilthead seabream (Sparus aurata).

Autor: Fuentes J; Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), Instituto de Ciencias Marinas de Andalucía, 11519, Puerto Real, Cádiz, Spain. xoan.fuentes@csic.es., Gregório SF; Centro de Ciências do Mar, Universidade do Algarve, 8005-139, Faro, Portugal., Fonseca F; ARNET (Aquatic Network, Associated Laboratory), Centre for Marine and Environmental Research (CIMA), University of Algarve, Faro, Portugal., Robles-Arozarena R; Testing Blue S.L., Calle Holanda 26, 11500, Puerto Real, Cádiz, Spain., Martos-Sitcha JA; Departamento de Biología Facultad de Ciencias del Mar y Ambientales, Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI-MAR), University of Cádiz, 11519, Puerto Real, Cádiz, Spain., Moyano FJ; Departamento de Biologia y Geologia Facultad de Ciencias, Campus de Excelencia Internacional del Mar (CEI-MAR), Universidad de Almeria, La Cañada de San Urbano, 04120, Almería, Spain. fjmoyano@ual.es.
Jazyk: angličtina
Zdroj: Fish physiology and biochemistry [Fish Physiol Biochem] 2024 Aug; Vol. 50 (4), pp. 1777-1790. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1007/s10695-024-01369-8
Abstrakt: In the context of modern aquaculture, the effort to reduce the reliance on fishmeal/marine ingredients in fish diets has led to the exploration of plant-based protein sources as potential substitutes, a dietary shift that disrupts the bile acid profile in fish. Therefore, bile salts are being sought as additives. However, artificially increased intestinal levels of bile acids may significantly impact mucosal function. Therefore, here, we explored the regulatory role in the intestine of gilthead sea bream (Sparus aurata) of (i) chenodeoxycholic acid (CDC), (ii) a mixture formed by two bile acids, 3% cholic acid and 97% deoxycholic acid (MIX), and (iii) a conjugated bile salt sodium taurocholate (TC) in Ussing chambers with the epithelial voltage clamp technique. We tested the bile salts in a 50-500 μg/ml concentration range, and all of them promoted ion absorption. Yet, clear concentration-dependent and more pronounced effects on the ion transport were observed in the posterior intestine. On the other hand, bile salts had no or minor effects on tissue resistance. However, there are indications that the MIX could have adverse effects at high concentrations (500 μg/ml), promoting a threefold increase in tissue permeability measured using FITC-dextran (4 kD) regardless of the intestinal region, thus suggesting an alteration in intestinal permeability at high bile salt concentrations. The findings from our study emphasize the importance of considering intestinal function when contemplating the possible use of a particular bile salt as a dietary supplement. It appears that bile salts, whether acting individually or in combination, play a pivotal role in orchestrating nutrient absorption by influencing the function of epithelial ion transport. However further research is needed to fully grasp the region-dependent nuances of bile salt effects on ion transport and the ultimate consequences for nutrient absorption in the context of fish aquaculture.
(© 2024. The Author(s).)
Databáze: MEDLINE
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