Characterization of pre-diapause phase in the northern Drosophila species D. ezoana.

Autor: Vaze KM; Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, USA. vazekm@umich.edu., Manoli G; Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany., Helfrich-Förster C; Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany.
Jazyk: angličtina
Zdroj: Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology [J Comp Physiol A Neuroethol Sens Neural Behav Physiol] 2024 Nov; Vol. 210 (6), pp. 901-908. Date of Electronic Publication: 2024 Jun 25.
DOI: 10.1007/s00359-024-01707-4
Abstrakt: Drosophila ezoana is a virilis group Drosophila species inhabiting northern latitudes. The flies enter adult reproductive diapause to survive winter upon exposure to short photoperiod conditions (short-day) over several consecutive days. Insect pre-diapause phase - the duration between the beginning of exposure to short days and expression of diapause is thought to be comprised of two distinct phases - (a) photoperiodic time measurement that detects short-days, followed by (b) physiological events leading to the expression of diapause phenotype. A short-day dependent segment of the pre-diapause phase thus approximates the process of photoperiodic time measurement. Continuous darkness has been found to be a neutral condition with respect to diapause regulation in many insect species. The effect of variable number of short-days followed by continuous darkness on diapause incidence thus allows identification of short-day dependent segment of pre-diapause phase thereby mapping the process of photo-periodic time measurement. Although, few weeks of exposure to short-days in adult stage is known to be sufficient for the expression of diapause in D. ezoana, the number of short days required for the completion of photo-periodic time measurement has never been systematically analysed. Our experiments show that continuous darkness is a neutral condition for diapause regulation also in D. ezoana. We utilized the neutral nature of continuous darkness to map the process of photoperiodic time measurement in the D. ezoana strain 124OJ8 which showed that integration of short-day photic cues over the first 10 days of pre-diapause phase is essential for diapause induction.
Competing Interests: Declarations Ethics Our studies do not require ethics committee approval. Conflict of interest The authors declare no conflict of interest. Competing interests CH-F is in the editorial board of Journal of Comparative Physiology A.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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