Generation of Oviductal Glycoprotein 1 Cre Mouse Model for the Study of Secretory Epithelial Cells of the Oviduct.

Autor:	McGlade EA; Obstetrics, Gynecology and Women's Health, University of Missouri-Columbia, Columbia, MO 65211, USA.; Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA., Mao J; Obstetrics, Gynecology and Women's Health, University of Missouri-Columbia, Columbia, MO 65211, USA., Stephens KK; Obstetrics, Gynecology and Women's Health, University of Missouri-Columbia, Columbia, MO 65211, USA., Kelleher AM; Obstetrics, Gynecology and Women's Health, University of Missouri-Columbia, Columbia, MO 65211, USA., Maddison LA; Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA., Bernhardt ML; Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA., DeMayo FJ; Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA., Lydon JP; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA., Winuthayanon W; Obstetrics, Gynecology and Women's Health, University of Missouri-Columbia, Columbia, MO 65211, USA.; Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA.
Jazyk:	angličtina
Zdroj:	Endocrinology [Endocrinology] 2024 May 27; Vol. 165 (7).
DOI:	10.1210/endocr/bqae070
Abstrakt:	The epithelial cell lining of the oviduct plays an important role in oocyte pickup, sperm migration, preimplantation embryo development, and embryo transport. The oviduct epithelial cell layer comprises ciliated and nonciliated secretory cells. The ciliary function has been shown to support gamete and embryo movement in the oviduct, yet secretory cell function has not been well characterized. Therefore, our goal was to generate a secretory cell-specific Cre recombinase mouse model to study the role of the oviductal secretory cells. A knock-in mouse model, Ovgp1Cre:eGFP, was created by expressing Cre from the endogenous Ovgp1 (oviductal glycoprotein 1) locus, with enhanced green fluorescent protein (eGFP) as a reporter. EGFP signals were strongly detected in the secretory epithelial cells of the oviducts at estrus in adult Ovgp1Cre:eGFP mice. Signals were also detected in the ovarian stroma, uterine stroma, vaginal epithelial cells, epididymal epithelial cells, and elongated spermatids. To validate recombinase activity, progesterone receptor (PGR) expression was ablated using the Ovgp1Cre:eGFP; Pgrf/f mouse model. Surprisingly, the deletion was restricted to the epithelial cells of the uterotubal junction (UTJ) region of Ovgp1Cre:eGFP; Pgrf/f oviducts. Deletion of Pgr in the epithelial cells of the UTJ region had no effect on female fecundity. In summary, we found that eGFP signals were likely specific to secretory epithelial cells in all regions of the oviduct. However, due to a potential target-specific Cre activity, validation of appropriate recombination and expression of the gene(s) of interest is absolutely required to confirm efficient deletion when generating conditional knockout mice using the Ovgp1Cre:eGFP line. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. See the journal About page for additional terms.)
Databáze:	MEDLINE
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