A stargate mechanism of Microviridae genome delivery unveiled by cryogenic electron tomography.

Autor:	Bardy P; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG., MacDonald CIW; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom., Kirchberger PC; Department of Microbiology & Molecular Genetics, Oklahoma State University, US., Jenkins HT; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG., Botka T; Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00, Brno, Czech Republic., Byrom L; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG.; Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Alim NTB; Department of Microbiology and Immunology, University of British Columbia, Vancouver, V6T 1Z3, BC, Canada., Traore DAK; Materials and Structural Analysis, Thermo Fisher Scientific, Achtseweg Noord 5, 5651 GG, Eindhoven, The Netherlands., König HC; Department of Microbiology and Immunology, University of British Columbia, Vancouver, V6T 1Z3, BC, Canada., Nicholas TR; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG., Chechik M; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG., Hart SJ; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom., Turkenburg JP; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom., Blaza JN; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG., Beatty JT; Department of Microbiology and Immunology, University of British Columbia, Vancouver, V6T 1Z3, BC, Canada., Fogg PCM; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG.; Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Antson AA; York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.; York Biomedical Research Institute, University of York, York, United Kingdom. YO10 5NG.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 11. Date of Electronic Publication: 2024 Jun 11.
DOI:	10.1101/2024.06.11.598214
Abstrakt:	Single-stranded DNA bacteriophages of the Microviridae family are major components of the global virosphere. Microviruses are highly abundant in aquatic ecosystems and are prominent members of the mammalian gut microbiome, where their diversity has been linked to various chronic health disorders. Despite the clear importance of microviruses, little is known about the molecular mechanism of host infection. Here, we have characterized an exceptionally large microvirus, Ebor, and provide crucial insights into long-standing mechanistic questions. Cryogenic electron microscopy of Ebor revealed a capsid with trimeric protrusions that recognise lipopolysaccharides on the host surface. Cryogenic electron tomography of the host cell colonized with virus particles demonstrated that the virus initially attaches to the cell via five such protrusions, located at the corners of a single pentamer. This interaction triggers a stargate mechanism of capsid opening along the 5-fold symmetry axis, enabling delivery of the virus genome. Despite variations in specific virus-host interactions among different Microviridae family viruses, structural data indicate that the stargate mechanism of infection is universally employed by all members of the family. Startlingly, our data reveal a mechanistic link for the opening of relatively small capsids made out of a single jelly-roll fold with the structurally unrelated giant viruses. Competing Interests: Conflict of interest D.A.K.T. is an employee of Thermo Fisher Scientific, a company dedicated to advancing electron cryo-imaging technology. Other authors declare no conflict of interest.
Databáze:	MEDLINE
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