How should we sample snail microbiomes?
| Autor: | Carruthers LV; School of Infection and Immunity, University of Glasgow, Glasgow G12 8TA, UK., Nordmeyer SC; Texas Biomedical Research Institute, PO Box 760549, San Antonio, TX 78258., Anderson TJ; Texas Biomedical Research Institute, PO Box 760549, San Antonio, TX 78258., Chevalier FD; Texas Biomedical Research Institute, PO Box 760549, San Antonio, TX 78258., Clec'h WL; Texas Biomedical Research Institute, PO Box 760549, San Antonio, TX 78258. |
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| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 11. Date of Electronic Publication: 2024 Jun 11. |
| DOI: | 10.1101/2024.06.11.598555 |
| Abstrakt: | Background: The microbiome is increasingly recognized to shape many aspects of its host biology and is a key determinant of health and disease. The microbiome may influence transmission of pathogens by their vectors, such as mosquitoes or aquatic snails. We previously sequenced the bacterial 16S V4 ribosomal DNA of the hemolymph (blood) of Biomphalaria spp. snails, one of the vectors of the human blood fluke schistosome. We showed that snail hemolymph harbored an abundant and diverse microbiome. This microbiome is distinct from the water environment and can discriminate snail species and populations. As hemolymph bathes snail organs, we then investigated the heterogeneity of the microbiome in these organs. Results: We dissected ten snails for each of two different species ( B. alexandrina and B. glabrata ) and collected their organs (ovotestis, hepatopancreas, gut, and stomach). We also ground in liquid nitrogen four whole snails of each species. We sampled the water in which the snails were living (environmental controls). Sequencing the 16S V4 rDNA revealed organ-specific microbiomes. These microbiomes harbored a lower diversity than the hemolymph microbiome, and the whole-snail microbiome. The organ microbiomes tend to cluster by physiological function. In addition, we showed that the whole-snail microbiome is more similar to hemolymph microbiome. Conclusions: These results are critical for future work on snail microbiomes and show the necessity of sampling individual organ microbiomes to provide a complete description of snail microbiomes. Competing Interests: Competing interests: The authors declare that they have no competing interests. |
| Databáze: | MEDLINE |
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