Increased biofilm formation in dual-strain compared to single-strain communities of Cutibacterium acnes.

Autor: Bjerg CSB; Department of Biomedicine, Faculty of Health, Aarhus University, Aarhus, Denmark.; Department of Biology, Section for Microbiology, Aarhus University, Aarhus, Denmark., Poehlein A; Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, Germany., Bömeke M; Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Göttingen, Germany., Himmelbach A; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany., Schramm A; Department of Biology, Section for Microbiology, Aarhus University, Aarhus, Denmark., Brüggemann H; Department of Biomedicine, Faculty of Health, Aarhus University, Aarhus, Denmark. brueggemann@biomed.au.dk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jun 24; Vol. 14 (1), pp. 14547. Date of Electronic Publication: 2024 Jun 24.
DOI: 10.1038/s41598-024-65348-y
Abstrakt: Cutibacterium acnes is a known opportunistic pathogen in orthopedic implant-associated infections (OIAIs). The species of C. acnes comprises distinct phylotypes. Previous studies suggested that C. acnes can cause single- as well as multi-typic infections, i.e. infections caused by multiple strains of different phylotypes. However, it is not known if different C. acnes phylotypes are organized in a complex biofilm community, which could constitute a multicellular strategy to increase biofilm strength and persistency. Here, the interactions of two C. acnes strains belonging to phylotypes IB and II were determined in co-culture experiments. No adverse interactions between the strains were observed in liquid culture or on agar plates; instead, biofilm formation in both microtiter plates and on titanium discs was significantly increased when combining both strains. Fluorescence in situ hybridization showed that both strains co-occurred throughout the biofilm. Transcriptome analyses revealed strain-specific alterations of gene expression in biofilm-embedded cells compared to planktonic growth, in particular affecting genes involved in carbon and amino acid metabolism. Overall, our results provide first insights into the nature of dual-type biofilms of C. acnes, suggesting that strains belonging to different phylotypes can form biofilms together with additive effects. The findings might influence the perception of C. acnes OIAIs in terms of diagnosis and treatment.
(© 2024. The Author(s).)
Databáze: MEDLINE
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