Validation and screening of patulin in apple beverages marketed in Morocco.

Autor: El Filali Z; Laboratory of Human Pathologies Biology, Faculty of Sciences, Mohammed V University in Rabat, Morocco; Regional Laboratory of Analysis and Research, Casablanca, Morocco., El Idrissi Boutaher A; Regional Laboratory of Analysis and Research, Casablanca, Morocco., Oumato J; Food Science Department, Agronomy and Veterinary Institute, Rabat, Morocco., Abdelmoumen H; Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnology, Biodiversity and Environment, Faculty of Sciences, Mohammed V University in Rabat, Morocco., Bourais I; Laboratory of Human Pathologies Biology, Faculty of Sciences, Mohammed V University in Rabat, Morocco; Mohammed VI University of Sciences and Health (UM6SS), Casablanca, Morocco; Mohammed VI Center for Research and Innovation (CM6RI), Rabat, Morocco. Electronic address: i.bourais@um5r.ac.ma.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Oct 30; Vol. 456, pp. 139994. Date of Electronic Publication: 2024 Jun 05.
DOI: 10.1016/j.foodchem.2024.139994
Abstrakt: Patulin is one of the mycotoxins frequently detected in apples and derivatives, representing a major food safety risk. This study aimed to validate a high-performance liquid chromatography (HPLC) method with an ultraviolet (UV) detector for patulin quantification and assess its occurrence in apple beverages marketed in Morocco. The validation parameters showed satisfactory results with adequate linearity (R > 0.997), a relative standard deviation below 2.5%, repeatability between 3.6 and 7.1%, reproducibility between 3.9 and 11.5%, a limit of quantification (LOQ) of 4 μg/L, and recoveries close to 100% for three levels. Analysis of 30 samples revealed patulin levels ranging from 0 to 16.36 μg/L, with 50% of samples showing negative levels. All positive results remained below the regulatory maximum limit of 50 μg/L. These findings affirm the efficacy of the HPLC proposed method in ensuring compliance with patulin regulations in apple beverages, underlining its importance in safeguarding food safety.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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