No detection of tick-borne encephalitis virus RNA in blood, urine or saliva of hospitalised immunocompetent tick-borne encephalitis patients.

Autor: Quarsten H; Department of Medical Microbiology, Sørlandet Hospital Trust, Kristiansand, Norway., Andreassen ÅK; Division for Infection Control and Environmental Health, Department of Virology, Norwegian Institute of Public Health, Oslo, Norway., Paulsen KM; Division for Infection Control and Environmental Health, Department of Virology, Norwegian Institute of Public Health, Oslo, Norway., Diekmann MJ; Division for Infection Control and Environmental Health, Department of Virology, Norwegian Institute of Public Health, Oslo, Norway., Eikeland R; The Norwegian National Advisory Unit on Tick-borne Diseases, Sørlandet Hospital Trust, Kristiansand, Norway.; Faculty of Health and Sport Science, University of Agder, Grimstad, Norway., Helleren R; Department of Clinical Medicine, Sørlandet Hospital Trust, Kristiansand, Norway., Bergström T; Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Noraas S; Department of Medical Microbiology, Sørlandet Hospital Trust, Kristiansand, Norway., Lorentzen ÅR; The Norwegian National Advisory Unit on Tick-borne Diseases, Sørlandet Hospital Trust, Kristiansand, Norway.; Department of Neurology, Sørlandet Hospital Trust, Kristiansand, Norway.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jun 24; Vol. 19 (6), pp. e0305603. Date of Electronic Publication: 2024 Jun 24 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0305603
Abstrakt: Tick-borne encephalitis (TBE) is usually diagnosed based on the presence of TBE virus (TBEV)-specific IgM and IgG antibodies in serum. However, antibodies induced by vaccination or cross-reactivity to previous flavivirus infections may result in false positive TBEV serology. Detection of TBEV RNA may be an alternative diagnostic approach to detect viral presence and circumvent the diagnostic difficulties present when using serology. Viral RNA in blood is commonly detectable only in the first viremic phase usually lasting up to two weeks, and not in the second neurologic phase, when the patients contact the health care system and undergo diagnostic work-up. TBEV RNA has previously been detected in urine in a few retrospective TBE cases in the neurologic phase, and furthermore RNA of other flaviviruses has been detected in patient saliva. In this study, blood, saliva and urine were collected from 31 hospitalised immunocompetent patients with pleocytosis and symptoms of aseptic meningitis and/or encephalitis, suspected to have TBE. We wanted to pursue if molecular testing of TBEV RNA in these patient materials may be useful in the diagnostics. Eleven of the 31 study patients were diagnosed with TBE based on ELISA detection of TBEV specific IgG and IgM antibodies. None of the study patients had TBEV RNA detectable in any of the collected patient material.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Quarsten et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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