Genetic features of BEL-1-producing and KPC-2-producing E. coli from hospital wastewater: human source or sewages adaptation.

Autor: Romero-Oraá L; Unidad Clínica de Enfermedades Infecciosas y Microbiología, Hospital Universitario Virgen Macarena, Instituto de Biomedicina de Sevilla IBIS, Seville, Spain., Pulido MR; Departamento de Microbiología, Universidad de Sevilla, Avda Dr. Fedriani S/N. 41009, Seville, Spain., Galán F; Unidad de Microbiología, Hospital Universitario Puerta del Mar, Cádiz, Spain., Palacios MVG; Servicio de Medicina Preventiva, Hospital Universitario Puerta del Mar, Cádiz, Spain., Pascual A; Unidad Clínica de Enfermedades Infecciosas y Microbiología, Hospital Universitario Virgen Macarena, Instituto de Biomedicina de Sevilla IBIS, Seville, Spain.; Departamento de Microbiología, Universidad de Sevilla, Avda Dr. Fedriani S/N. 41009, Seville, Spain.; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain., López-Cerero L; Unidad Clínica de Enfermedades Infecciosas y Microbiología, Hospital Universitario Virgen Macarena, Instituto de Biomedicina de Sevilla IBIS, Seville, Spain. llopez@us.es.; Departamento de Microbiología, Universidad de Sevilla, Avda Dr. Fedriani S/N. 41009, Seville, Spain. llopez@us.es.; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain. llopez@us.es.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2024 Jul; Vol. 31 (31), pp. 43896-43902. Date of Electronic Publication: 2024 Jun 24.
DOI: 10.1007/s11356-024-33875-w
Abstrakt: Hospital sewage is an ecosystem that facilitates the transfer of antibiotic and heavy metal resistance genes and the interaction of human and environmental bacteria. In this environment, we have detected the presence of 7 KPC-2 and BEL-1 co-producing E. coli isolates of two different clones over a 10-month period in the same hospital. All isolates carried bla KPC-2 and the operon mer on the same IncP plasmid of similar size and an IncN plasmid of different size each clone carrying bla BEL-1 . Both IncN-blaBEL-1 plasmids shared a 77 kb region containing bla BEL-1 alongside with fosE, bla OXA-10 and aac(6')-1b genes in a class 3 integron within a Tn3 transposon. The major IncN plasmid contained in addition a region homolog to P1-like bacteriophage RCS47, including the lytic RepL and lysogenic proteins, but other phage regions were incomplete. The characters such as the temporal persistence in sewage, the absence of colonized patients in the hospital or in the region, the presence of a p1 phage-plasmid fusion and the infrequent class 3 integron as genetic platform would indicate that BEL-1-producing isolates could have been generated in situ by adaptation to human sewage. Part of the microbiota in these discharges could be explained by the interactions of sewage ecosystems and not derive directly from the hospital.
(© 2024. The Author(s).)
Databáze: MEDLINE
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