| Autor: |
Roberts SH; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine., Alrata L; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine., Abdulsattar D; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine., Joseph K; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine., Abu-Amer W; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine., Zaghloul M; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine., Arif B; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine., Alhamad T; Department of Medicine, Division of Nephrology, Washington University School of Medicine., Remedi MS; Department of Medicine, Division of Endocrinology, Washington University School of Medicine., Lin Y; Department of Surgery, Section of Transplant Surgery, Washington University School of Medicine., Zayed MA; Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine; CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine; Division of Molecular Cell Biology, Washington University School of Medicine; Department of Biomedical Engineering, Washington University McKelvey School of Engineering; zayedm@wustl.edu. |
| Abstrakt: |
Pancreatic islet transplantation is an emerging treatment for type I diabetes; however, it is limited by donor matching and availability. Porcine islet xenotransplantation offers a promising alternative to allotransplantation, with the potential for large-scale production of on-demand, functional islets. The yield and viability of isolated islets is highly susceptible to the quality of the donor pancreas and the method of procurement, particularly the duration of warm-ischemia time. To improve organ preservation and subsequent islet yield and viability, we have developed a protocol for surgical perfusion and resection of the porcine pancreas. This protocol employs direct infrarenal aortic cannulation and organ perfusion to both minimize warm-ischemia time and simplify the procedure for operators who do not have extensive surgical expertise. Subsequent arterial perfusion of the pancreas via the aorta flushes stagnant blood from the microvasculature, thereby reducing thrombosis and oxidative damage to the tissue. This manuscript provides a detailed protocol for surgical perfusion and resection of the porcine pancreas, followed by islet isolation and purification. |
