Chemical Composition, Nutritional Value, Antioxidative, and In Vivo Anti-inflammatory Activities of Opuntia Stricta Cladode.

Autor:	Harrabi B; Research Unit UR 12 ES 13, Laboratory of Pharmacology Faculty of Medicine of Sfax, University of Sfax, SFax 3029, Tunisia., Ben Nasr H; Research Unit UR 12 ES 13, Laboratory of Pharmacology Faculty of Medicine of Sfax, University of Sfax, SFax 3029, Tunisia., Amri Z; Biochemistry Laboratory, LR12ES05 'Nutrition- Functional Foods and vascular Health', Faculty of Medicine, University of Monastir, Monastir 5019, Tunisia., Brahmi F; Department of Chemistry, College of Science, University of Ha'il, Ha'il 45851, Saudi Arabia., El Feki A; Research Unit UR 11 ES 72 - Biodiversity and Aquatic Ecosystems, Faculty of Sciences of Sfax, University of Sfax, Sfax 3000, Tunisia., Zeghal K; Research Unit UR 12 ES 13, Laboratory of Pharmacology Faculty of Medicine of Sfax, University of Sfax, SFax 3029, Tunisia., Ghozzi H; Research Unit UR 12 ES 13, Laboratory of Pharmacology Faculty of Medicine of Sfax, University of Sfax, SFax 3029, Tunisia., Siddiqui AJ; Laboratory of General Biology, Department of Biology, University of Ha'il, Ha'il 45851, Saudi Arabia., Adnan M; Laboratory of General Biology, Department of Biology, University of Ha'il, Ha'il 45851, Saudi Arabia., Aloufi B; Laboratory of General Biology, Department of Biology, University of Ha'il, Ha'il 45851, Saudi Arabia., Jilani S; Laboratory of General Biology, Department of Biology, University of Ha'il, Ha'il 45851, Saudi Arabia., Boufahja F; Department of Biology, College of Science, Imam Ibn Saud Islamic University, Riyadh 11652, Saudi Arabia., Badraoui R; Laboratory of General Biology, Department of Biology, University of Ha'il, Ha'il 45851, Saudi Arabia.; Section of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, La Rabta-Tunis 1007, Tunisia.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2024 Jun 08; Vol. 9 (24), pp. 26724-26734. Date of Electronic Publication: 2024 Jun 08 (Print Publication: 2024).
DOI:	10.1021/acsomega.4c04330
Abstrakt:	The cactus family plant has been used in folk medicine for a long time. In this work, Opuntia stricta chemical composition and its antioxidative and anti-inflammatory properties were investigated. Our results showed that O. stricta is highly rich in fibers and minerals. The present study assessed the levels of polyphenol contents and antioxidant and in vivo anti-inflammatory activities. The highest phenolic compounds and antioxidant activity were observed in the methanolic extract. Concerning the qualitative analysis, nine phenolic and organic acids were identified and quantified by high-performance liquid chromatography (HPLC). Luteolin-7-Glu (4.25 μg/g), apigenin-7-Glu (3.15 μg/g), and catechin (2.85 μg/g) were identified as major phenolic compounds. The predominant fatty acids detected by gas chromatography (GC) coupled to a flame ionization detector were linoleic and linolenic acids (35.11%). A factorial design plan was used to determine the effect of temperature, agitation speed, and maceration period on phenolic contents. In vivo , the methanol extract from Opuntia stricta showed anti-inflammatory activity. The computational modeling reveals that O. stricta compounds bind VEGF, IL-6, and TNF-α with high binding scores that reach -8.7 kcal/mol and establish significant molecular interactions with some key residues that satisfactorily explain both in vitro and in vivo findings. These data indicate that Opuntia stricta cladode powder could be potentially useful in pharmaceutical and food applications. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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