Microfragmented abdominal adipose tissue-derived stem cells from knee osteoarthritis patients aged 29-65 years demonstrate in vitro stemness and low levels of cellular senescence.

Autor: Hammer FA; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark., Hölmich P; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark., Nehlin JO; Department of Clinical Research Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark., Vomstein K; Department of Obstetrics and Gynecology, The Fertility Clinic Copenhagen University Hospital-Hvidovre Hvidovre Denmark., Blønd L; Department of Orthopedic Surgery Zealand University Hospital-Køge Køge Denmark., Hölmich LR; Department of Plastic Surgery Copenhagen University Hospital-Herlev and Gentofte Herlev Denmark., Barfod KW; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark., Bagge J; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark.
Jazyk: angličtina
Zdroj: Journal of experimental orthopaedics [J Exp Orthop] 2024 Jun 21; Vol. 11 (3), pp. e12056. Date of Electronic Publication: 2024 Jun 21 (Print Publication: 2024).
DOI: 10.1002/jeo2.12056
Abstrakt: Purpose: To investigate the level of cellular senescence in stem cells derived from microfragmented abdominal adipose tissue harvested from patients with knee osteoarthritis (OA).
Methods: Stem cells harvested from microfragmented abdominal adipose tissue from 20 patients with knee OA, aged 29-65 years (mean = 49.8, SD = 9.58), were analysed as a function of patient age and compared with control cells exhibiting signs of cellular senescence. Steady-state mRNA levels of a panel of genes associated with senescence were measured by qPCR. Intracellular senescence-associated proteins p16 and p21, and senescence-associated β-galactosidase activity were measured by flow cytometry. Cellular proliferation was assessed using a 5-ethynyl-2'-deoxyuridine proliferation assay. Stemness was assessed by stem cell surface markers using flow cytometry and the capacity to undergo adipogenic and osteogenic differentiation in vitro.
Results: No correlation was found between cellular senescence levels of the microfragmented adipose tissue-derived stem cells and patient age for any of the standard assays used to quantify senescence. The level of cellular senescence was generally low across all senescence-associated assays compared to the positive senescence control. Stemness was verified for all samples. An increased capacity to undergo adipogenic differentiation was shown with increasing patient age ( p  = 0.02). No effect of patient age was found for osteogenic differentiation.
Conclusions: Autologous microfragmented adipose tissue-derived stem cells may be used in clinical trials of knee OA of patients aged 29-65 years, at least until passage 4, as they show stemness potential and negligible senescence in vitro.
Level of Evidence: Not applicable.
Competing Interests: The authors declare no conflict of interest.
(© 2024 The Author(s). Journal of Experimental Orthopaedics published by John Wiley & Sons Ltd on behalf of European Society of Sports Traumatology, Knee Surgery and Arthroscopy.)
Databáze: MEDLINE
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