The resistance of hydrogenotrophic methanogenic microorganisms to ofloxacin in sludge anaerobic digestion process.

Autor: Wu Y; College of Environment and Resources, Xiangtan University, Xiangtan, 411105, China. Electronic address: wuyanxin@xtu.edu.cn., Wu C; College of Environment and Resources, Xiangtan University, Xiangtan, 411105, China., Liu X; College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China., Chen H; College of Environment and Resources, Xiangtan University, Xiangtan, 411105, China., Deng Z; College of Environment and Resources, Xiangtan University, Xiangtan, 411105, China., Wang D; College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China., Wu Z; School of Stomatology, Lanzhou University, Lanzhou, 730000, China., Tang S; Testing Technology Company of Changsha Research Institute of Mining and Metallurgy Co.,LTD, Changsha 410082, China., Huang X; College of Resources and Environment, Anqing Normal University, Anhui, 246011, China.
Jazyk: angličtina
Zdroj: Journal of environmental management [J Environ Manage] 2024 Aug; Vol. 365, pp. 121522. Date of Electronic Publication: 2024 Jun 22.
DOI: 10.1016/j.jenvman.2024.121522
Abstrakt: Ofloxacin (OFL) is a commonly used antibiotic that can enter wastewater treatment plants and be adsorbed by the sludge, resulting in a high OFL concentration in sludge and affecting the subsequent sludge anaerobic digestion process. However, the micro mechanisms involved in this process have not been thoroughly studied. Therefore, this study focuses on the effect of OFL on the sludge anaerobic digestion of sludge to provide such support. The experimental results showed that the maximal methane yield decreased from 277.7 to 164.7 mL/g VSS with the OFL concentration increased from 0 to 300 mg/L. Additionally, OFL hindered the intermediate biochemical processes of hydrolysis, acidogenesis, acetogenesis, and acetoclastic methanogenesis. However, it promoted hydrogenotrophic methanogenesis process, using H 2 as substrate, with the concentration of 300 mg/L OFL was 5.54 fold methane production of that in the control. Further investigation revealed that the negative effect of OFL was likely due to the induction of reactive oxygen species, which led to a decrease in cell activity and interference with the activity of key enzymes. Microbiological analysis revealed that OFL reduced the relative abundance of hydrolysis and acidogenesis bacteria, and Methanosaeta archaea, while increasing the relative abundance of hydrogenotrophic methanogenesis microorganism from 36.54% to 51.48% as the OFL concentration increase from 0 to 300 mg/L.
Competing Interests: Declaration of competing interest This is a new research article named “The resistance of hydrogenotrophic methanogenic microorganisms to ofloxacin in sludge anaerobic digestion process” and all the authors have reviewed the final version of the manuscript and approved to submit it to your journal. We guarantee that this manuscript will not be submitted elsewhere for publication while being considered by the Journal of Environmental Management, that it is not under consideration for publication elsewhere, that its publication is approved by all authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, it will not be published elsewhere in the same form, in English or in any other language, including electronically without the written consent of the copyright-holder. There are no conflicts of any interest, including employment, consultancies, stock ownership, honoraria, paid expert testimony, patent applications/registrations, and grants or other funding. I am very appreciated by your kindness, looking forward to hearing from you.
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Databáze: MEDLINE
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