YAP1 regulates esophageal stem cells' self-renewal and differentiation.

Autor: Choi YJ; Department of New Biology, DGIST, Daegu, 42988, South Korea; New Biology Research Center, DGIST, Daegu, 42988, South Korea., Myeong J; Department of New Biology, DGIST, Daegu, 42988, South Korea., Kim JH; Department of Life Science, Dongguk University, Gyeonggi-do, 10326, South Korea., Kim S; Department of New Biology, DGIST, Daegu, 42988, South Korea., Song K; Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, 03722, South Korea., Lee M; Department of Life Science, Dongguk University, Gyeonggi-do, 10326, South Korea., Jeong Y; Department of New Biology, DGIST, Daegu, 42988, South Korea; New Biology Research Center, DGIST, Daegu, 42988, South Korea. Electronic address: jyt@dgist.ac.kr.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Sep 24; Vol. 726, pp. 150280. Date of Electronic Publication: 2024 Jun 19.
DOI: 10.1016/j.bbrc.2024.150280
Abstrakt: Esophageal epithelium is one of the most proliferative and regenerative epithelia in our body, indicating robust stem cell activity. However, the underlying mechanisms regulating the self-renewal and differentiation of esophageal stem cells need to be more elucidated. Here, we identify the role of YAP1 in esophageal stem cells. YAP1 is differentially expressed in the nuclei of esophageal basal cells. Furthermore, the treatment of verteporfin, a YAP1 inhibitor, interfered with esophageal organoid formation. Consistently, YAP1 deletion decreased esophageal organoid formation and the expression of basal genes while increasing the expression of suprabasal genes. Finally, global transcriptomic analysis revealed that YAP1 inhibition induced a significant enrichment of gene sets related to keratinization and cornification, while depleting gene sets related to DNA repair and chromosome maintenance. Our data uncover a novel regulatory mechanism for esophageal stem cells, which could provide a potential strategy for esophageal regenerative medicine.
Competing Interests: Declaration of competing interest The authors declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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