Gga-miR-200a-3p suppresses avian reovirus-induced apoptosis and viral replication via targeting GRB2.

Autor: Zhao Y; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zhou L; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zheng H; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Gao L; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Cao H; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Li X; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zheng SJ; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Wang Y; National Key Laboratory of Veterinary Public Health Security, China; Animal Epidemiology of the Ministry of Agriculture, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: vetwyq@cau.edu.cn.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Aug; Vol. 295, pp. 110149. Date of Electronic Publication: 2024 Jun 12.
DOI: 10.1016/j.vetmic.2024.110149
Abstrakt: Avian reovirus (ARV) is a significant pathogen that causes various clinical diseases in chickens, including viral arthritis, chronic respiratory diseases, retarded growth, and malabsorption syndrome. These conditions result in substantial economic losses for the global poultry industry. MicroRNAs (miRNAs), a type of small noncoding RNAs that regulate gene expression post transcriptionally by silencing or degrading their RNA targets, play crucial roles in response to pathogenic infections. In this study, transfection of DF-1 cells with gga-miR-200a-3p, an upregulated miRNA observed in ARV-infected cells, significantly suppressed ARV-induced apoptosis by directly targeting GRB2 and impeded ARV replication. Conversely, knockdown of endogenous gga-miR-200a-3p in DF-1 cells using a specific miRNA inhibitor enhanced ARV-induced apoptosis and promoted GRB2 expression, thereby facilitating viral growth within cells. Consistently, inhibition of GRB2 activity through siRNA-mediated knockdown reduced viral titers. Therefore, gga-miR-200a-3p plays a vital antiviral role in the host response to ARV infection by suppressing apoptosis via direct targeting of GRB2 protein. This information enhances our understanding of the mechanisms by which host cells combat against ARV infection through self-encoded small RNA molecules and expands our knowledge regarding the involvement of microRNAs in the host response to pathogenic infections.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Yongqiang Wang reports financial support was provided by National Natural Science Foundation of China and Earmarked Fund for Modern Agro-Industry Technology Research System of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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