Bacteriophage ISP eliminates Staphylococcus aureus in planktonic phase, but not in the various stages of the biofilm cycle.

Autor: Verheul M; Department of Infectious Diseases, Leiden University Medical Center, 2300RC, Leiden, The Netherlands. m.verheul@lumc.nl.; Department of Orthopedics, Leiden University Medical Center, 2300RC, Leiden, The Netherlands. m.verheul@lumc.nl., Mulder AA; Department of Cell and Chemical Biology, Electron Microscopy Facility, Leiden University Medical Center, 2300RC, Leiden, The Netherlands., van Dun SCJ; Department of Infectious Diseases, Leiden University Medical Center, 2300RC, Leiden, The Netherlands.; Department of Orthopedics, Leiden University Medical Center, 2300RC, Leiden, The Netherlands., Merabishvili M; Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium., Nelissen RGHH; Department of Orthopedics, Leiden University Medical Center, 2300RC, Leiden, The Netherlands., de Boer MGJ; Department of Infectious Diseases, Leiden University Medical Center, 2300RC, Leiden, The Netherlands., Pijls BG; Department of Orthopedics, Leiden University Medical Center, 2300RC, Leiden, The Netherlands., Nibbering PH; Department of Infectious Diseases, Leiden University Medical Center, 2300RC, Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jun 22; Vol. 14 (1), pp. 14374. Date of Electronic Publication: 2024 Jun 22.
DOI: 10.1038/s41598-024-65143-9
Abstrakt: Metal-implant associated bacterial infections are a major clinical problem due to antibiotic treatment failure. As an alternative, we determined the effects of bacteriophage ISP on clinical isolates of Staphylococcus aureus in various stages of its life cycle in relation to biofilm formation and maturation. ISP effectively eliminated all planktonic phase bacteria, whereas its efficacy was reduced against bacteria attached to the metal implant and bacteria embedded within biofilms. The biofilm architecture hampered the bactericidal effects of ISP, as mechanical disruption of biofilms improved the efficacy of ISP against the bacteria. Phages penetrated the biofilm and interacted with the bacteria throughout the biofilm. However, most of the biofilm-embedded bacteria were phage-tolerant. In agreement, bacteria dispersed from mature biofilms of all clinical isolates, except for LUH15394, tolerated the lytic activity of ISP. Lastly, persisters within mature biofilms tolerated ISP and proliferated in its presence. Based on these findings, we conclude that ISP eliminates planktonic phase Staphylococcus aureus while its efficacy is limited against bacteria attached to the metal implant, embedded within (persister-enriched) biofilms, and dispersed from biofilms.
(© 2024. The Author(s).)
Databáze: MEDLINE
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