An integrated strategy for quality control of Pseudobulbus Cremastrae seu Pleiones based on Q-marker.

Autor: Wang J; National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry Co., Ltd., China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China; School of Pharmacy, Fudan University, Shanghai 201203, China., Wu M; National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry Co., Ltd., China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China., Liu J; Shuguang Hospital affiliated with Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China., Mao X; National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry Co., Ltd., China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China., Cui W; Shijiazhuang Ke-di Pharmaceutical Co., Ltd., Shijiazhuang 050090, China., Lei C; School of Pharmacy, Fudan University, Shanghai 201203, China., Huang C; National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry Co., Ltd., China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China., Hu X; National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry Co., Ltd., China State Institute of Pharmaceutical Industry, No. 285, Gebaini Road, Shanghai 201203, China. Electronic address: xjtuyxhx@126.com.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Aug 16; Vol. 1730, pp. 465105. Date of Electronic Publication: 2024 Jun 17.
DOI: 10.1016/j.chroma.2024.465105
Abstrakt: Pseudobulbus Cremastrae seu Pleiones (PCsP), a traditional Chinese medicine known as ‶Shan-Ci-Gu″, possesses properties for clearing heat, counteracting toxicity, dissipating phlegm, and resolving masses. As a TCM with multiple bases, the dried pseudobulbs of Pleione bulbocodioides (PB), Pleione yunnanensis (PY) and Cremastra appendiculata (CA) are considered to be the official sources of PCsP. Additionally, several unofficial substitutes are also available in the market. To enhance the quality control of PCsP, an integrated strategy based on Q-marker was proposed. Initially, a study of integrating plant metabolomics, target isolation, structure identification, and activity testing afforded five Q-markers, including three new compounds. Furthermore, a quality evaluation method using a single standard to determine multi-components (SSDMC) based on Q-marker was established, which could effectively distinguish PB from CA and the counterfeit herbs. Finally, the transitivity of Q-markers was explored through a representative Chinese compound prescription containing PCsP. The results indicated that the identified Q-markers together with the established analysis methods could be effectively applied for quality control of PCsP and its preparations.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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